iMatrix-511 SILK Stem Cell Culture Substrate

Catalog NP892-021
Size 6 x 175 µg available
Price
$455.00
$455.00
Availability In stock
Catalog
NP892-021

Recombinant Laminin-511 SILK E8-Fragments

iMatrix-511 SILK is xeno-free, recombinant laminin-511 E8 fragments, produced in recombinant silkworm cocoon. 

iMatrix-511 SILK's features make it an ideal matrix for pluripotent stem cell culture:

  • Promotes greater stem cell adhesion than all other matrix proteins that have been tested
  • Xeno-free formulation / insect cell production
  • Easy to use (liquid format)
  • E8 fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-511
  • Equivalent performance, but lower cost than the legacy iMatrix-511 product

Laminin is localized to the basement membrane, and plays a key role in cell adhesion and proliferation. Laminin-511 (α5, β1 laminin) binds to integrin α6β1 to promote cell signaling. Laminin-511 provides an ideal matrix for the proliferation of a wide variety of cell types including stem and iPS cells.

iMatrix-511 SILK is functionally equivalent to iMatrix-511 (NP892-011, NP892-012).  The only difference is the expression system used for bioproduction.  The iMatrix-511 SILK product is produced in recombinant silkworm cocoon, and the iMatrix-511 legacy product is produced in CHO-S cells.  Both are E8 fragments that have been purified using the same processes.


Product Specifications

Product Name
iMatrix-511 SILK Stem Cell Culture Substrate
Catalog Number
NP892-021
Size
6 x 175 µg available
Molecular Weight
150 kDa
Purity
>95% pure
Formulation
Purified Laminin-511 E8 proteolytic fragment
Storage and Stability
Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.
Quality Control
Integrin binding Kd < 10 nM
Sterility
sterile
Notice To Purchaser
REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market
Recommended Usage
iMatrix-511 is suitable for use as a substrate for culture of various cell types, including ES/iPS cells.
Concentration
500 µg/mL
Source
Transgenic SILKWORM Cocoon
Manufacturer
Matrixome Corporation (Japan)

PRODUCT SPECIFICATION SHEET

PRODUCT DESCRIPTIION
  • iMatrix Product Flyer

References

  1. Takayama K. et al., "Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells". Biochemical and Biophysical Research Commun. 474 (1): 91-96 (2016).
  2. Nishimura K. et al., "Estradial facilitates functional integration of iPSC-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin a5b1".  Stem Cell Reports 6 (4): 511-524 (2016).
  3. Matsuno K. et al., "Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stemcells". Differentiation 2016.04.002.
  4. Hayashi R. et al., "Co-ordinated ocular development ofrom human iPS cells and recovery of corneal function". Nature 531, 368-80 (2016),
  5. Sasaki K. et al., "Robust in vitro induction of human germ celll fate from pluripotent stem cells". Cell Stem Cell 17 (2):178-194 (2015).
  6. Okumura N. et al., "Laminin-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells". Invest Ophthalmal Vis Sci. 56 (5), 2933-42 (2015).
  7. Nakagawa M. et al., "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells:. Scientific Reports 4: 3594 (2014).
  8. Miyazaki T. et al. "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotentn stem cells.  Nature Communications 3: 1236 (2012).
  9. Taniguchi Y. et al., "The C-terminal region of laminin beta-chains modulates the integrin-binding affinities of laminins:" J. Biol. Chem. 284 (12): 7820-31 (2009).
  10. Ido H. et al., "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by lamiinins.  J. Biol. Chem. 282 (15): 11144-54 (2017).

Product Specifications

Product Name
iMatrix-511 SILK Stem Cell Culture Substrate
Catalog Number
NP892-021
Size
6 x 175 µg available
Molecular Weight
150 kDa
Purity
>95% pure
Formulation
Purified Laminin-511 E8 proteolytic fragment
Storage and Stability
Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.
Quality Control
Integrin binding Kd < 10 nM
Sterility
sterile
Notice To Purchaser
REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market
Recommended Usage
iMatrix-511 is suitable for use as a substrate for culture of various cell types, including ES/iPS cells.
Concentration
500 µg/mL
Source
Transgenic SILKWORM Cocoon
Manufacturer
Matrixome Corporation (Japan)

PRODUCT SPECIFICATION SHEET

PRODUCT DESCRIPTIION
  • iMatrix Product Flyer

References

  1. Takayama K. et al., "Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells". Biochemical and Biophysical Research Commun. 474 (1): 91-96 (2016).
  2. Nishimura K. et al., "Estradial facilitates functional integration of iPSC-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin a5b1".  Stem Cell Reports 6 (4): 511-524 (2016).
  3. Matsuno K. et al., "Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stemcells". Differentiation 2016.04.002.
  4. Hayashi R. et al., "Co-ordinated ocular development ofrom human iPS cells and recovery of corneal function". Nature 531, 368-80 (2016),
  5. Sasaki K. et al., "Robust in vitro induction of human germ celll fate from pluripotent stem cells". Cell Stem Cell 17 (2):178-194 (2015).
  6. Okumura N. et al., "Laminin-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells". Invest Ophthalmal Vis Sci. 56 (5), 2933-42 (2015).
  7. Nakagawa M. et al., "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells:. Scientific Reports 4: 3594 (2014).
  8. Miyazaki T. et al. "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotentn stem cells.  Nature Communications 3: 1236 (2012).
  9. Taniguchi Y. et al., "The C-terminal region of laminin beta-chains modulates the integrin-binding affinities of laminins:" J. Biol. Chem. 284 (12): 7820-31 (2009).
  10. Ido H. et al., "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by lamiinins.  J. Biol. Chem. 282 (15): 11144-54 (2017).