iMatrix-411 Endothelial Cell Substrate

Catalog NP892-041
NP892-042
Size 2x175 µg and 6x175 µg available
Price
$495.00
$495.00
Availability In stock
Catalog
NP892-04

Recombinant Laminin-411 E8-Fragments

iMatrix-411's features make it an ideal matrix for some cell culture applications:

  • Xeno-free formulation / CHO-S cell bioproduction
  • Easy to use (liquid format)
  • E8-fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-411
  • Cultivation of iPSC on iMatrix-411 induces a cell fate transition to endothelial progenitor cells

iMatrix-411 is a highly purified and refined product of human recombinant laminin-411 (E8 fragment) expressed by CHO-S cells.  Laminin-411 is found predominantly expressed in the vascular endothelial basement membrane(1) and in Langerhans islet capillary endothelial cells of the pancreas(2). 

As a cell culture ECM-substrate iMatrix-411 can assist in establishing human endothelial cell primary cultures from fresh human bio-specimens.

Human iPS cells, when cultured on iMatrix-411, are robustly induced (>95%) to differentiate into endothelial progenitor cells with angiogenesis capacity(1).  Other published reports have used iMatrix-411 and iMatrix-511 in a step-wise protocol to generate cholangiocytes capable of forming 3D cyst-structures from iPSC-derived hepatoblastic cells(3).


Product Specifications

Product Name
iMatrix-411 Endothelial Cell Substrate
Catalog Number
NP892-04
Size
2x175 µg and 6x175 µg available
Molecular Weight
150 kDa
Purity
>95% pure
Formulation
Purified Laminin-411 E8 proteolytic fragment
Storage and Stability
Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.
Quality Control
Activity: Kd for integrin binding <10 nM.
Sterility
Sterile
Notice To Purchaser
REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market
Recommended Usage
iMatrix-411 is suitable for use as a substrate for culture of various cell types, but most notably for vascular endothelial cells.
Concentration
500 µg/mL
Source
CHO-S cell expression
Manufacturer
Matrixome Corporation (Japan)

SPECIFICATION SHEET


References

  1. Ohta R. et al., "Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells".  Scientific Reports 6:35680 / DOI: 10.1038/srep35680 (2016).
  2. Lammert E., Cleaver O., Melton D., Mechanisms of Development (Elsevier) 120 (1) 59-64 (2003).
  3. Takayama K. et al., "Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells". Biochemical and Biophysical Research Commun. 474 (1): 91-96 (2016).
  4. Nishimura K. et al., "Estradial facilitates functional integration of iPSC-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin a5b1".  Stem Cell Reports 6 (4): 511-524 (2016).
  5. Matsuno K. et al., "Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stemcells". Differentiation 2016.04.002.
  6. Hayashi R. et al., "Co-ordinated ocular development ofrom human iPS cells and recovery of corneal function". Nature 531, 368-80 (2016),
  7. Sasaki K. et al., "Robust in vitro induction of human germ celll fate from pluripotent stem cells". Cell Stem Cell 17 (2):178-194 (2015).
  8. Okumura N. et al., "Laminin-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells". Invest Ophthalmal Vis Sci. 56 (5), 2933-42 (2015).
  9. Nakagawa M. et al., "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells:. Scientific Reports 4: 3594 (2014).
  10. Miyazaki T. et al. "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotentn stem cells.  Nature Communications 3: 1236 (2012).
  11. Taniguchi Y. et al., "The C-terminal region of laminin beta-chains modulates the integrin-binding affinities of laminins:" J. Biol. Chem. 284 (12): 7820-31 (2009).
  12. Ido H. et al., "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by lamiinins.  J. Biol. Chem. 282 (15): 11144-54 (2017).

Product Specifications

Product Name
iMatrix-411 Endothelial Cell Substrate
Catalog Number
NP892-04
Size
2x175 µg and 6x175 µg available
Molecular Weight
150 kDa
Purity
>95% pure
Formulation
Purified Laminin-411 E8 proteolytic fragment
Storage and Stability
Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.
Quality Control
Activity: Kd for integrin binding <10 nM.
Sterility
Sterile
Notice To Purchaser
REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market
Recommended Usage
iMatrix-411 is suitable for use as a substrate for culture of various cell types, but most notably for vascular endothelial cells.
Concentration
500 µg/mL
Source
CHO-S cell expression
Manufacturer
Matrixome Corporation (Japan)

SPECIFICATION SHEET

References

  1. Ohta R. et al., "Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells".  Scientific Reports 6:35680 / DOI: 10.1038/srep35680 (2016).
  2. Lammert E., Cleaver O., Melton D., Mechanisms of Development (Elsevier) 120 (1) 59-64 (2003).
  3. Takayama K. et al., "Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells". Biochemical and Biophysical Research Commun. 474 (1): 91-96 (2016).
  4. Nishimura K. et al., "Estradial facilitates functional integration of iPSC-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin a5b1".  Stem Cell Reports 6 (4): 511-524 (2016).
  5. Matsuno K. et al., "Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stemcells". Differentiation 2016.04.002.
  6. Hayashi R. et al., "Co-ordinated ocular development ofrom human iPS cells and recovery of corneal function". Nature 531, 368-80 (2016),
  7. Sasaki K. et al., "Robust in vitro induction of human germ celll fate from pluripotent stem cells". Cell Stem Cell 17 (2):178-194 (2015).
  8. Okumura N. et al., "Laminin-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells". Invest Ophthalmal Vis Sci. 56 (5), 2933-42 (2015).
  9. Nakagawa M. et al., "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells:. Scientific Reports 4: 3594 (2014).
  10. Miyazaki T. et al. "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotentn stem cells.  Nature Communications 3: 1236 (2012).
  11. Taniguchi Y. et al., "The C-terminal region of laminin beta-chains modulates the integrin-binding affinities of laminins:" J. Biol. Chem. 284 (12): 7820-31 (2009).
  12. Ido H. et al., "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by lamiinins.  J. Biol. Chem. 282 (15): 11144-54 (2017).