iMatrix-221 Cardiac & Myoblast Cell Culture Substrate

Catalog NP892-061
NP892-062
Size 2x175 µg and 6x175 µg available
Price
$495.00
$495.00
Availability In stock
Catalog
NP892-06

Recombinant Laminin-221 E-8 Fragments

iMatrix-221's features make it an ideal matrix for some cell culture applications:

  • Xeno-free formulation / CHO-S cell bioproduction
  • Easy to use (liquid format)
  • E8-fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-221

iMatrix-221 is a highly purified and refined product of human recombinant laminin-221 (E8 fragment) expressed by CHO-S cells.  Laminin-221 is found predominantly in the basal lamina of striated muscle tissues.  Expression deficiencies are implicated in muscular dystophy and cardiomyopathies (1). 

Laminins of the alpha-2 type are also a predomiant form found in the human adult heart tissue (2).  Cultivation of iPSC-drived cardiomyocytes on iMatrix-221 has been demonstrated to maintain strong contractility in attached monolayer culture (3). 


Product Specifications

Product Name
iMatrix-221 Cardiac & Myoblast Cell Culture Substrate
Catalog Number
NP892-06
Size
2x175 µg and 6x175 µg available
Molecular Weight
150 kDa
Purity
>95% pure
Formulation
Purified Laminin-221 E8 proteolytic fragment
Storage and Stability
Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.
Quality Control
Activity: Kd for integrin binding <10 nM.
Sterility
Sterile
Notice To Purchaser
REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market.
Recommended Usage
iMatrix-221 is suitable for use as a substrate for culture of various myoblast cell types, but most notably for cardiomyocytes.
Concentration
500 µg/mL
Source
CHO-S cell expression
Manufacturer
Matrixome Corporation (Japan)

SPECIFICATION SHEET

  • iMatrix-221 Product Spec Sheet

  1. Oliviero P. et.al., "Expression of laminin alpha2 chain during normal and pathological growth of myocardiu in rat and human". Cardiovascular Research 46, 346-355 (2000).
  2. Ja K.P. Myu Mia et.al., "iPSC-derived human cardiac progenitor cells improve ventricular remodelling via angiogenesis and interstitial networking of infarcted myocardium". J Cell Mol Med 20(2), 323-332 (2016).
  3. unpublished, Osaka University (2018).
  4. Miyazaki T. et al. "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotentn stem cells.  Nature Communications 3: 1236 (2012).
  5. Taniguchi Y. et al., "The C-terminal region of laminin beta-chains modulates the integrin-binding affinities of laminins:" J. Biol. Chem. 284 (12): 7820-31 (2009).
  6. Ido H. et al., "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by lamiinins.  J. Biol. Chem. 282 (15): 11144-54 (2017).

Product Specifications

Product Name
iMatrix-221 Cardiac & Myoblast Cell Culture Substrate
Catalog Number
NP892-06
Size
2x175 µg and 6x175 µg available
Molecular Weight
150 kDa
Purity
>95% pure
Formulation
Purified Laminin-221 E8 proteolytic fragment
Storage and Stability
Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.
Quality Control
Activity: Kd for integrin binding <10 nM.
Sterility
Sterile
Notice To Purchaser
REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market.
Recommended Usage
iMatrix-221 is suitable for use as a substrate for culture of various myoblast cell types, but most notably for cardiomyocytes.
Concentration
500 µg/mL
Source
CHO-S cell expression
Manufacturer
Matrixome Corporation (Japan)

SPECIFICATION SHEET

  • iMatrix-221 Product Spec Sheet
  1. Oliviero P. et.al., "Expression of laminin alpha2 chain during normal and pathological growth of myocardiu in rat and human". Cardiovascular Research 46, 346-355 (2000).
  2. Ja K.P. Myu Mia et.al., "iPSC-derived human cardiac progenitor cells improve ventricular remodelling via angiogenesis and interstitial networking of infarcted myocardium". J Cell Mol Med 20(2), 323-332 (2016).
  3. unpublished, Osaka University (2018).
  4. Miyazaki T. et al. "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotentn stem cells.  Nature Communications 3: 1236 (2012).
  5. Taniguchi Y. et al., "The C-terminal region of laminin beta-chains modulates the integrin-binding affinities of laminins:" J. Biol. Chem. 284 (12): 7820-31 (2009).
  6. Ido H. et al., "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by lamiinins.  J. Biol. Chem. 282 (15): 11144-54 (2017).