Neuronal assays

For more than 15 years, Prof Stefan Przyborski - our Chief Scientific Officer and Durham University Professor of Cell Technology - has been using Alvetex™ 3D scaffold technology to develop 3D cell assays. This includes neuronal aggregates, which are used in our neurite outgrowth model for studying the effects of compounds and conditions on neurite formation.

 

Video clip showing calcium signals flowing through our human cortical neurons in vitro. 


3D Neurite Outgrowth Model

By measuring neurite outgrowth you can assess the effects of your test articles on neurite formation or toxicity. Our translational neurite outgrowth model combines our award-winning Alvetex scaffold technology with iPSC-derived human neurons to produce a mature, neuronal network that develops dense synaptic connections.

Developed in partnership with the University of Durham, REPROCELL’s iPSC-derived StemRNA™ Neuro cells - available as healthy wild-type and Alzheimer Disease patient-derived (presenilin 2 mutation) - are used to assess pathological changes in vitro. This system is compatible with many physiological and pathological ECM coatings (laminin, CSPGs), known pathway inhibitors, and therapeutic lead compounds.

  • Measure inhibition and recovery of neurite outgrowth
  • Use iPSC-derived StemRNA™ Neuro cells derived from healthy or Alzheimer Disease donors 
  • Explore pathological changes in functional human cells in vitro 
  • Assess physiological and pathological ECM coatings, known pathway inhibitors, and test articles

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Immunocytochemistry analysis showing neurites in our 3D outgrowth model. 


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