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Gastrointestinal Motility

Our organ bath model can be used to investigate the effect of your test article on gut motility. These studies can determine how drugs affect motility in a therapeutic capacity (e.g. to prevent diarrhea or to treat constipation, gastroparesis, or disorders of gastrointestinal sphincters or gallbladder) or from a safety perspective. Full-thickness circular or longitudinal gastrointestinal muscle strips can be used to investigate the effect of your test article.

wire-myograph-01

Diagram of the organ bath model we use to measure Gastrointestinal motility. 


Intestinal motility 

Changes in intestinal motility can lead to irritable bowel syndrome, diarrhea, and Ulcerative Colitis. In our electrical field stimulated organ bath model, we can measure changes in gastrointestinal motility in response to your test compound. The specific results that will be provided are the effects of increasing concentrations of test articles on the contractile state of isolated human intestinal tissue.

  • Galantamine and Meptazinol are available as reference compounds
  • Suggested testing on your compound is in duplicate at 6 concentrations
  • Responses shall be expressed as % change from baseline EFS response
100_1122-1An example of healthy intestinal tissue used in our gut motility studies.

Gut motility (ACh-esterase)
This model uses electrically stimulated tissues to assess whether your test article affects GI motility compared with Galantamine.

Gut motility (ACh esterase) model →

Gut motility (opioid receptors)
This model uses electrically stimulated tissues to assess whether your test article affects small or large intestine motility.

Gut motility (Opioid) model →

 


Gastric motility 

Movement of the stomach is essential for the mixing of the stomach contents with gastric juices and to move the partially digested food from the stomach into the intestines. Using electrically stimulated human stomach tissue, we can assess whether your test articles cause a change in contractile force.

  • Erythromycin is used as a control compound
  • Effect on the Motilin receptor measured via changes in contractile response 
  • Electrical field stimulation organ bath model used 
  • Test articles tested in duplicate at 6 concentrations.

Stomach tissue

An example of fresh stomach tissue used for our gastric motility studies.

Stomach motility (motilin)
This assay investigates whether your test article causes a change in contractile force in electrically stimulated stomach tissue. 

Stomach motility (motilin) model →


Lower esophageal sphincter motility 

The primary function of the lower esophageal sphincter (LES) is to allow food transit from the esophagus into the stomach. An incompetent LES can lead to Gastroesophageal reflux disease, increasing the risk for metaplasia and esophageal adenocarcinoma.

Human LES relaxation
This assay uses the lower esophageal sphincter from a healthy donor to assess the effect of your test article on sphincter relaxation.

Relaxation of LES model →

Human LES contraction 
This assay uses the lower esophageal sphincter from a healthy donor to assess the effect of your test article on sphincter contraction.

Contraction of LES model →

 

Interested in finding out more about our gastrointestinal motility assays? Fill in the form to speak with a human tissue expert.