REPROCELL USA and REPROCELL Europe will be closed for the holidays starting Wednesday 12/25/2019. We will reopen on Thursday 1/2/2020. Please place orders by Wednesday 12/18/2019 to ensure delivery before 2020.

Stemgent Stemfactor FGF-basic, Human Recombinant

Code: 03-0002-base
  • $137.00
  • (Delivery from $41.50)
Available | Usually dispatched within 24 hours
Prices apply to customers in North America only
Loading...
Be the first to review Stemgent Stemfactor FGF-basic, Human Recombinant. Write a review

Fibroblast Growth Factor-basic (bFGF), also known as FGF-2, is a heparin-binding member of the FGF superfamily of molecules. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues by promoting cellular proliferation and differentiation. Additionally, bFGF is a critical component of embryonic stem cell culture medium, allowing cells to remain in an undifferentiated state in serum-free medium1,2. Stemfactor bFGF is an approximate 17 kDa protein consisting of 155 amino acid residues.

Product Name

Stemfactor FGF-basic, Human Recombinant

Catalog Number

03-0002

Size

50 µg

Purity

Greater than 95% by SDS-PAGE analysis

Formulation

Lyophilized from 5 mM Tris-HCl, pH 7.5 with 150 mM NaCl.

Reconstitution

Centrifuge briefly and then reconstitute bFGF in 500 µL of 10 mM Tris, pH 7.6, to yield a stock solution of 0.1 mg/mL of bFGF. Avoid freeze-thaw cycles as it can result in loss of activity.

Storage and Stability

Stemfactor bFGF is shipped at room temperature. Lyophilized bFGF is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C. Reconstituted bFGF, at concentrations greater than or equal to 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and up to 6 months when stored at −80 °C.

Sterility

Tested to be negative forMycoplasmasp. by PCR and microbial contamination by a sterility test.

Source

E. coli

Amino Acid Sequence

MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS

Uniprot Accession Number

P09038, residues 1-155

Endotoxin Level

Less than 1.0 EU/µg of bFGF as determined by the LAL method.

Biologic Activity

The ED50 is 0.1 to 1 ng/mL as determined by the dose dependent proliferation of NIH 3T3 cells.

  1. Amit, M., Carpenter, M.K., Inokuma, M.S., Chiu, C.P., Harris, C.P., Waknitz, M.A., Itskovitz-Eldor, J., and Thompson, J.A. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227: 271-278 (2000).
  2. Xu, C., Rosler, E., Jiang, J., Lebkowski, J.S., Gold, J.D., O'Sullivan, C., Delavan-Boorsma, K., Mok, M., Bronstein, A., and Carpenter, M.K. Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium. Stem Cells 23: 315-323 (2005).
  3. Vallier, L., Alexander, M., and Pedersen, R.A.  Activin/Nodal and FGF pathways corporate to maintain pluripotency of human embryonic stem cells. J Cell Sci 118: 4495-4509 (2005).
  4. Wang, G., Zhang, H., Zhao, Y., Li, J., Cai, J., Wang, P., Meng, S., Feng, J., Miao, C., Ding, M., Li, D., and Deng, H. Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun 330: 934-942 (2005).
  5. Greber, B., Lehrach, H., and Adjaye, J. Fibroblast growth factor 2 modulates transforming growth factor β signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal. Stem Cells 25: 455-464 (2007).

Additional Publications

Top