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Stemgent Stemfactor Activin A, Human Recombinant

Code: 03-0001-base
  • $158.00
  • (Delivery from $41.50)
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Stemfactor Activin A is a crystallography-grade preparation providing the highest purity available. Activin A is bi-modal in action having been shown to maintain pluripotency of stem cells and promote differentiation1,2,3,4. Mature recombinant human Activin A is a ~25 kDa disulfide-linked homodimer of two 116 amino acid residue βA subunits.

Stemgent conveniently provides all the components comprising the 5i/L/A media supplement necessary to support naïve pluripotent stem cell applications.

5i (inhibitors):

L: Stemfactor LIF, Human Recombinant (03-0016), and

A: Stemfactor Activin A, Human Recombinant (03-0001)

Product Name

Stemfactor Activin A, Human Recombinant

Catalog Number

03-0001

Size

5 µg

Purity

Greater than 98% by SDS-PAGE analysis

Formulation

Lyophilized from 20% acetonitrile

Reconstitution

Centrifuge briefly and reconstitute Activin A in 10 mM HCl before dilution in cell culture medium. Avoid freeze-thaw cycles as it can result in loss of activity.

Storage and Stability

Activin A is shipped at room temperature. Lyophilized Activin A is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C. Reconstituted Activin A, at concentrations greater than 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and for 1 year when stored at −80 °C.

Source

E. coli

Amino Acid Sequence

GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

Uniprot Accession Number

P08476, residues 311-426

Endotoxin Level

Less than 1.0 EU/µg of Activin A as determined by the LAL method.

Biologic Activity

The ED50 is less than 6 ng/ml as determined by its ability to inhibit the proliferation of mouse MPC-11 cells.

Specification Sheets

Safety Data Sheets

  1. Beattie, G.M., Lopez, A.D., Bucay, N., Hinton, A., Firpo, M.T., King, C.C., and Hayek, A. Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells 23: 489-495 (2005).
  2. Vallier, L., Alexander, M., and Pedersen, R.A. Activin/Nodal and FGF pathways corporate to maintain pluripotency of human embryonic stem cells. J Cell Sci 118: 4495-4509(2005).
  3. Sulzbacher, S., Schroeder, I.S., Truong, T.T., and Wobus, A.M. Activin A-induced differentiation of embryonic stem cells into endoderm and pancreatic progenitors- the influence of differentiation factors and culture conditions. Stem Cell Rev 5: 159-173(2009). 
  4. Valdimarsdottir, G., and Mummery, C. Functions of the TGFβ superfamily in human embryonic cells. APMIS 113: 773-789(2005).

Additional Publications

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