Feeder Cells (MEF)

Catalog RCHEFC003
Size 3x10⁶ cells x 5 vials
Price Price on Request
$0.00
Availability In stock
Catalog
RCHEFC003

Very low passage mouse embryonic fibroblast feeder cells. Hugely popular cell type for feeder-dependent culture of iPSC from a wide range of species.

MEF (Mouse Embryonic Fibroblasts) feeder cells are applicable for a wide range of ES/iPS cell culture. ReproCELL offers exceptionally consistent quality thanks to rigorous validation testing. See protocol (below) for more information.

Features

  • Mouse Embryonic Fibroblast feeder cells derived from mouse embryo.
  • A feeder cell line applicable for culture of mouse, primate, human ES/iPS cells.
  • Validated high quality with every lot.
  • Mitomycin C-treated cells ready for use.

Product Specifications

Product Name
Feeder Cells (MEF)
Catalog Number
RCHEFC003
Size
3x10⁶ cells x 5 vials
Storage and Stability
Liquid nitrogen. This product is very thermosensitive. Upon delivery please move the cell vials into liquid nitrogen to ensure optimal cell attachment levels.


References

  • Chen, Ling, et al. "Mouse induced pluripotent stem cell microenvironment generates epithelial-mesenchymal transition in mouse Lewis lung cancer cells." American journal of cancer research 4.1 (2014): 80.
  • Kim, Mee‐Hae, Eri Masuda, and Masahiro Kino‐Oka. "Kinetic analysis of deviation from the undifferentiated state in colonies of human induced pluripotent stem cells on feeder layers." Biotechnology and bioengineering (2014).
  • Haraguchi, Yuji, et al. "Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering." Journal of tissue engineering and regenerative medicine (2013).
  • Sugimoto, Kouji, et al. "Effects of hypoxia on pluripotency in murine iPS cells." Microscopy research and technique 76.10 (2013): 1084-1092.
  • Chen, Ling, et al. "A model of cancer stem cells derived from mouse induced pluripotent stem cells." PLoS One 7.4 (2012): e33544.
  • Fukushima, H., et al. "SSEA-4 is a marker of human deciduous periodontal ligament stem cells." Journal of dental research 91.10 (2012): 955-960.
  • Imai, Koichi, et al. "In vitro study of cell differentiation by two type mouse embryo stem cells on mono-and multilayer nanocarbon tubes." Applied Surface Science 258.22 (2012): 8444-8447.
  • Ishikawa, Tetsuya, Keitaro Hagiwara, and Takahiro Ochiya. "Generation and hepatic differentiation of human iPS cells." Liver Stem Cells. Springer New York, (2012). 103-114.
  • Masuda, Shigeo, et al. "A simplified in vitro teratoma assay for pluripotent stem cells injected into rodent fetal organs." Cell Medicine 3.1-3 (2012): 1-3.
  • IMAI, Koichi, et al. "An Attempt to Cell Differentiation in Three-dimensional Culture System Using Non-feeder ES-D3 Cells and Feeder Layer Type ES cells." Journal of Oral Tissue Engineering 8.3 (2011): 203-211.
  • Inamura, Mitsuru, et al. "Efficient generation of hepatoblasts from human ES cells and iPS cells by transient overexpression of homeobox gene HEX." Molecular Therapy 19.2 (2011): 400-407.
  • Kobayashi, Hideyuki. "Pluripotent Stem Cells Induced from Testicular Tissue of a Man with Klinefelter Syndrome (47, XXY) by Four Transcription Factors (OCT4, SOX2, KLF4, and C-MYC)." (2011).
  • Kobayashi, Hideyuki, et al. "Reprogramming of adult human testicular cells by four transcription factors (OCT4, SOX2, KLF4, and C-MYC)." Reproductive medicine and biology 10.2 (2011): 105-112.
  • Sakai, Y., and K. Nakazawa. "Novel Microchip Technique for the Transfer of Spheroids as Floating Cultures to Micropatterned-Adherent Cultures." J Biochip Tissue chip S 4 (2011): 2153-0777.
  • Sakai, Yusuke, Yukiko Yoshiura, and Kohji Nakazawa. "Embryoid body culture of mouse embryonic stem cells using microwell and micropatterned chips." Journal of bioscience and bioengineering 111.1 (2011): 85-91.
  • Oka, Y., et al. "293FT cells transduced with four transcription factors (OCT4, SOX2, NANOG, and LIN28) generate aberrant ES-like cells." J Stem cell Regenerative Med 3 (2010): 149-56.
  • Lee, Tae-Hee, et al. "Functional recapitulation of smooth muscle cells via induced pluripotent stem cells from human aortic smooth muscle cells."Circulation research 106.1 (2010): 120-128
  • Sakai, Yusuke, et al. "Effect of microwell chip structure on cell microsphere production of various animal cells." Journal of bioscience and bioengineering110.2 (2010): 223-229.

Product Specifications

Product Name
Feeder Cells (MEF)
Catalog Number
RCHEFC003
Size
3x10⁶ cells x 5 vials
Storage and Stability
Liquid nitrogen. This product is very thermosensitive. Upon delivery please move the cell vials into liquid nitrogen to ensure optimal cell attachment levels.

References

  • Chen, Ling, et al. "Mouse induced pluripotent stem cell microenvironment generates epithelial-mesenchymal transition in mouse Lewis lung cancer cells." American journal of cancer research 4.1 (2014): 80.
  • Kim, Mee‐Hae, Eri Masuda, and Masahiro Kino‐Oka. "Kinetic analysis of deviation from the undifferentiated state in colonies of human induced pluripotent stem cells on feeder layers." Biotechnology and bioengineering (2014).
  • Haraguchi, Yuji, et al. "Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering." Journal of tissue engineering and regenerative medicine (2013).
  • Sugimoto, Kouji, et al. "Effects of hypoxia on pluripotency in murine iPS cells." Microscopy research and technique 76.10 (2013): 1084-1092.
  • Chen, Ling, et al. "A model of cancer stem cells derived from mouse induced pluripotent stem cells." PLoS One 7.4 (2012): e33544.
  • Fukushima, H., et al. "SSEA-4 is a marker of human deciduous periodontal ligament stem cells." Journal of dental research 91.10 (2012): 955-960.
  • Imai, Koichi, et al. "In vitro study of cell differentiation by two type mouse embryo stem cells on mono-and multilayer nanocarbon tubes." Applied Surface Science 258.22 (2012): 8444-8447.
  • Ishikawa, Tetsuya, Keitaro Hagiwara, and Takahiro Ochiya. "Generation and hepatic differentiation of human iPS cells." Liver Stem Cells. Springer New York, (2012). 103-114.
  • Masuda, Shigeo, et al. "A simplified in vitro teratoma assay for pluripotent stem cells injected into rodent fetal organs." Cell Medicine 3.1-3 (2012): 1-3.
  • IMAI, Koichi, et al. "An Attempt to Cell Differentiation in Three-dimensional Culture System Using Non-feeder ES-D3 Cells and Feeder Layer Type ES cells." Journal of Oral Tissue Engineering 8.3 (2011): 203-211.
  • Inamura, Mitsuru, et al. "Efficient generation of hepatoblasts from human ES cells and iPS cells by transient overexpression of homeobox gene HEX." Molecular Therapy 19.2 (2011): 400-407.
  • Kobayashi, Hideyuki. "Pluripotent Stem Cells Induced from Testicular Tissue of a Man with Klinefelter Syndrome (47, XXY) by Four Transcription Factors (OCT4, SOX2, KLF4, and C-MYC)." (2011).
  • Kobayashi, Hideyuki, et al. "Reprogramming of adult human testicular cells by four transcription factors (OCT4, SOX2, KLF4, and C-MYC)." Reproductive medicine and biology 10.2 (2011): 105-112.
  • Sakai, Y., and K. Nakazawa. "Novel Microchip Technique for the Transfer of Spheroids as Floating Cultures to Micropatterned-Adherent Cultures." J Biochip Tissue chip S 4 (2011): 2153-0777.
  • Sakai, Yusuke, Yukiko Yoshiura, and Kohji Nakazawa. "Embryoid body culture of mouse embryonic stem cells using microwell and micropatterned chips." Journal of bioscience and bioengineering 111.1 (2011): 85-91.
  • Oka, Y., et al. "293FT cells transduced with four transcription factors (OCT4, SOX2, NANOG, and LIN28) generate aberrant ES-like cells." J Stem cell Regenerative Med 3 (2010): 149-56.
  • Lee, Tae-Hee, et al. "Functional recapitulation of smooth muscle cells via induced pluripotent stem cells from human aortic smooth muscle cells."Circulation research 106.1 (2010): 120-128
  • Sakai, Yusuke, et al. "Effect of microwell chip structure on cell microsphere production of various animal cells." Journal of bioscience and bioengineering110.2 (2010): 223-229.