NutriStemR XF/FF

2015.03.18 Enhanced mRNA reprogramming

≪The Stemgent mRNA Reprogramming Kit is the fastest, safest, and most efficient method for generating integration-free, virus-free, clinically relevant human iPS cells.≫ ≪Features≫  ・Non-integrating — no risk of genomic integration  ・With robust reprogramming, colonies can emerge and be picked up in as early as 14 days  ・Stoichiometric control of individual reprogramming factors  ・Eliminates need for multi-step passaging and screening of colonies  ・Validated protocol using normal and diseased cells ≪Overview≫ Fast gneneration of gootprint-gree iPS cell lines With fast reprogramming kinetics and non-viral application, reprogramming with mRNA produces clinically-relevant iPS cell lines months earlier than other systems that require additional work to screen new cells for the presents of contaminating viral […]

2015.03.17 Self-Replicative RNA reprogramming (blood cell reprogramming)

A Self- Replicative RNA reprogramming system optimized for Endothelial Progenitor Cells (EPCs), makes blood- to- iPSC conversion reproducible in labs. Features  ・No genomic integration  ・Virus-free  ・Feeder-free workflow  ・Reprograms Endothelial Progenitor Cells (EPCs) derived from whole blood  ・Optimized reprogramming protocol + EPC derivation protocol  ・Only 2 transfections required Overview Only 2 transfections required! Select iPSC colonies in less than a month! Pluripotency immunocytochemistry (ICC) of EPC-srRNA-iPS cell line derived from human EPCs. Nuclear pluripotency staining with Rex1, Nanog, and Oct4 antibodies is seen in red. Cell surface pluripotency staining with SSEA4, TRA-1-81, and TRA-1-60 antibodies is seen in green. Merged images are shown. Primary reprogramming culture morphology progression, resulting from the […]

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