2015.03.17 Self-Replicative RNA reprogramming (blood cell reprogramming)

A Self- Replicative RNA reprogramming system optimized for Endothelial Progenitor Cells (EPCs), makes blood- to- iPSC conversion reproducible in labs. Features  ・No genomic integration  ・Virus-free  ・Feeder-free workflow  ・Reprograms Endothelial Progenitor Cells (EPCs) derived from whole blood  ・Optimized reprogramming protocol + EPC derivation protocol  ・Only 2 transfections required Overview Only 2 transfections required! Select iPSC colonies in less than a month! Pluripotency immunocytochemistry (ICC) of EPC-srRNA-iPS cell line derived from human EPCs. Nuclear pluripotency staining with Rex1, Nanog, and Oct4 antibodies is seen in red. Cell surface pluripotency staining with SSEA4, TRA-1-81, and TRA-1-60 antibodies is seen in green. Merged images are shown. Primary reprogramming culture morphology progression, resulting from the […]

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