Human Nanog Polyclonal Antibody

Rabbit anti-Human Nanog Polyclonal Antibody

Transcriptional factors, OCT3/4 (POU5F1) and STAT3 function as key regulators in maintaining pluripotency of stem cells. Thus, POU5F1 and STAT3 have been widely used as molecular markers of pluripotential stem cells. Pluripotential cell-specific Nanog gene is a newly identified homeodomain-bearing transcriptional factor. Importantly, Nanog is expressed specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Therefore, Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human.

human Nanog antibody
Immunogen Human Nanog peptide
Raised in Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified
Species specificity Human specific
Cross reactivity No cross reactivity
Concentration 0.20 mg/mL
Tested applications Western blot
Immunocytochemistry
Application notes WB: Use at a dilution of 1/300 – 1/2000
Immunocytochemistry: Use at a dilution of 1/150 – 1/700
Storage buffer Phosphate buffered saline incl. 0.1% sodium azide
Storage Store at +4°C for short term use. Aliquot and store at -20°C or -80°C.
Avoid repeated freeze / thaw cycles.

 

human Nanog antibody immunostaining

IF using hNanog antibody
Cells: Human embryonic stem cells, grown on mouse embryonic fibroblasts.

human Nanog antibody western blot

Western blot using hNanog antibody

 

Price list
Catalog No. Product Retail Price Quantity Purchase
RCAB003P Anti human Nanog antibody (200uL) $500.00Contact the distributor of your regionChoose your country.To be shipped from Japan. Please inquire.Unavailable at this time.
RCAB004P-F Anti human Nanog antibody (100uL) $300.00Contact the distributor of your regionChoose your country.To be shipped from Japan. Please inquire.Unavailable at this time.
Payment method can be chosen from Paypal / credit card / bank transfer.

 

Data Sheets

The data sheet for each lot of the product (open in a new window)