Stemolecule™ ec23 (ATRA Alt.)
Product Classification : Small molecule / CytokinesResearch Theme : Differentiation
ATRA supports the growth and differentiation of neural cells in culture, but as with other naturally occurring retinoids it is unstable and isomerizes (degrades) readily when exposed to light.
To overcome this problem Reinnervate has developed ec23® – a chemically-stable and light-stable alternative to ATRA that has similar biological activity and does not degrade
A major challenge in stem cell biology is the ability to control the development of cells and tissues in a predictable way. Biotechnologists require molecules that induce reliable and reproducible biological activity resulting in consistent modes of cell differentiation.
ATRA (all-trans retinoic acid) is well known for its ability to regulate cell
differentiation. However it has a short shelf-life because it absorbs light and degrades into isomers. Cell cultures containing ATRA are inconsistent, leading to problems with reproducibility of experimental results. To address this issue Reinnervate developed ec23® as a pan-RAR receptor agonist that is stable but maintains the same biological activity as ATRA.
Human TERA2.cl.SP12 EC cells exposed to either ec23® or ATRA form populations of terminally
differentiated neurons after 21 days. Immunocytochemical staining for neurofilament-200 was performed on differentiated cultures and the number of positive cells quantified. Results show that ec23® is a more potent inducer of neurogenesis than ATRA. In direct comparison to ATRA, synthetic retinoid ec23® induces larger numbers of neural cells with far less variability and with the added advantage of compound stability. See Christie et al. 2008, Org Biomol Chem, v6, p3497.