Self-Replicative RNA reprogramming (blood cell reprogramming)

Product Classification : RNA Reprogramming for hiPSCResearch Theme : ES/iPS cells

Stemgent Brand

A Self- Replicative RNA reprogramming system optimized for Endothelial Progenitor Cells (EPCs), makes blood- to- iPSC conversion reproducible in labs.

Features

 ・No genomic integration
 ・Virus-free
 ・Feeder-free workflow
 ・Reprograms Endothelial Progenitor Cells (EPCs) derived from whole blood
 ・Optimized reprogramming protocol + EPC derivation protocol
 ・Only 2 transfections required


Overview

Only 2 transfections required! Select iPSC colonies in less than a month!

Pluripotency immunocytochemistry (ICC) of EPC-srRNA-iPS cell line derived from human EPCs. Nuclear pluripotency staining with Rex1, Nanog, and Oct4 antibodies is seen in red. Cell surface pluripotency staining with SSEA4, TRA-1-81, and TRA-1-60 antibodies is seen in green. Merged images are shown.

Primary reprogramming culture morphology progression, resulting from the reprogramming of peripheral blood derived EPCs with Stemgent StemRNA-SR Reprogramming Kit (Cat. No. 00-0075). iPS cell morphologies emerge as early as Day 14 and can be isolated by Day 26. Day 14 and Day 21 primary iPS cell colonies were identified using Stemgent StainAlive™ TRA-1-60 antibody.

Histological analysis of teratoma resulting from the injection of EPC-srRNA-iPS cells into the kidney capsule of NOD-Scid mice.

Normal karyotype of EPC-srRNA-iPS cell line expanded on Corning Matrigel with NutriStem XF/FF Culture Medium, which was confirmed by passage9.


Product list

Catalog No. Product Quantity Price
00-0075

Stemgent StemRNA-SR Reprogramming Kit

Content
  • OKSiM srRNA
  • microRNA Reprogramming Cocktail
  • B18R Recombinant Protein
Ask
01-0005 NutriStemR XF/FF 500 mL Ask
RCHEOT002 bFGF 25 μg Ask
09-0068 StainAlive™ TRA-1-60 Antibody (DyLight™ 488), Mouse anti-Human 100 μL Ask
*five wells in a standard 6-well plate format *Requires hypoxic growth condition (5% O2)

Presentations

  • Yoshioka, N. et al. (2013) Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell; 13(2): 246-54.
  • Geti, I. et al. (2012) A practical and efficient cellular substrate for the generation of induced pluripotent stem cells from adults: blood-derived endothelial progenitor cells. Stem Cells Transl Med.; 1:855-65.