Self-Replicative RNA reprogramming (blood cell reprogramming)
Product Classification : RNA Reprogramming for hiPSCResearch Theme : ES/iPS cells
A Self- Replicative RNA reprogramming system optimized for Endothelial Progenitor Cells (EPCs), makes blood- to- iPSC conversion reproducible in labs.
Pluripotency immunocytochemistry (ICC) of EPC-srRNA-iPS cell line derived from human EPCs. Nuclear pluripotency staining with Rex1, Nanog, and Oct4 antibodies is seen in red. Cell surface pluripotency staining with SSEA4, TRA-1-81, and TRA-1-60 antibodies is seen in green. Merged images are shown.
Primary reprogramming culture morphology progression, resulting from the reprogramming of peripheral blood derived EPCs with Stemgent StemRNA-SR Reprogramming Kit (Cat. No. 00-0075). iPS cell morphologies emerge as early as Day 14 and can be isolated by Day 26. Day 14 and Day 21 primary iPS cell colonies were identified using Stemgent StainAlive™ TRA-1-60 antibody.
Histological analysis of teratoma resulting from the injection of EPC-srRNA-iPS cells into the kidney capsule of NOD-Scid mice.
Normal karyotype of EPC-srRNA-iPS cell line expanded on Corning Matrigel with NutriStem XF/FF Culture Medium, which was confirmed by passage9.
Stemgent StemRNA-SR Reprogramming KitContent
|01-0005||NutriStemR XF/FF||500 mL||Ask|
|09-0068||StainAlive™ TRA-1-60 Antibody (DyLight™ 488), Mouse anti-Human||100 μL||Ask|
- Yoshioka, N. et al. (2013) Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell; 13(2): 246-54.
- Geti, I. et al. (2012) A practical and efficient cellular substrate for the generation of induced pluripotent stem cells from adults: blood-derived endothelial progenitor cells. Stem Cells Transl Med.; 1:855-65.