Enhanced mRNA reprogramming
Product Classification : RNA Reprogramming for hiPSCResearch Theme : ES/iPS cells
≪The Stemgent mRNA Reprogramming Kit is the fastest, safest, and most efficient method for generating integration-free, virus-free, clinically relevant human iPS cells.≫
Fast gneneration of gootprint-gree iPS cell lines
With fast reprogramming kinetics and non-viral application, reprogramming with mRNA produces clinically-relevant iPS cell lines months earlier than other systems that require additional work to screen new cells for the presents of contaminating viral remnants.
The total time needed to generate a characterized iPS cell line using virus-based systems can take up to 25 weeks, whereas the Stemgent mRNA Reprogramming System generates virus-free, integration-free iPS cell lines in less than 2 weeks. This system enables the generation of fully characterized and banked iPS cell lines ready to use in as little as 9 weeks
Highly efficient and Safe, virus-free reprogramming
The Stemgent mRNA Reprogramming System provides efficiencies greater than 1% as compared to other methods, which yield reprogramming efficiencies varying from 0.00001 to 0.01%. In addition to increased yield of colonies and fast reprogramming kinetics, mRNA reprogramming does not require laborious multi-step passaging or screening for viral or genomic integration once the new colonies are derived.
The mRNA Reprogramming System eliminates virus bio-containment and safety issues, and carries no risk for insertional mutagenesis, an inherent concern with DNA-based reprogramming methods.
mRNA Reprogramming KitContent
microRNA Booster KitContent
Stemgent Stemfect RNA™ Transfection KitContent
|01-0005||NutriStemR XF/FF||500 mL||Ask|
|GSC-3002G||Nuff cell||4-5×106 cells||Ask|
|09-0068||StainAlive™ TRA-1-60 Antibody (DyLight™ 488), Mouse anti-Human||100 μL||Ask|
|09-0069||StainAlive™ TRA-1-81 Antibody (DyLight™ 488), Mouse anti-Human||100 μL||Ask|
Safety Data Sheet
- Mandal PK, Rossi DJ. (2013) Reprogramming human fibroblasts to pluripotency using modified mRNA. Nature Protocols. 8(3):568-82
- Angel M, Yanik MF. (2010) Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins. PLoS One. 5(7):e11756
- Warren L, et al. (2010) Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell. 5;7(5):618-30.
- Yakubov E, et al. (2010) Reprogramming of human fibroblasts to pluripotent stem cells using mRNA of four transcription factors. Biochem Biophys Res Commun. 394(1):189-93.