Enhanced mRNA reprogramming

Product Classification : RNA Reprogramming for hiPSCResearch Theme : ES/iPS cells

Enhanced
summary

≪The Stemgent mRNA Reprogramming Kit is the fastest, safest, and most efficient method for generating integration-free, virus-free, clinically relevant human iPS cells.≫


≪Features≫

 ・Non-integrating — no risk of genomic integration
 ・With robust reprogramming, colonies can emerge and be picked up in as early as 14 days
 ・Stoichiometric control of individual reprogramming factors
 ・Eliminates need for multi-step passaging and screening of colonies
 ・Validated protocol using normal and diseased cells


≪Overview≫

Fast gneneration of gootprint-gree iPS cell lines

With fast reprogramming kinetics and non-viral application, reprogramming with mRNA produces clinically-relevant iPS cell lines months earlier than other systems that require additional work to screen new cells for the presents of contaminating viral remnants.
The total time needed to generate a characterized iPS cell line using virus-based systems can take up to 25 weeks, whereas the Stemgent mRNA Reprogramming System generates virus-free, integration-free iPS cell lines in less than 2 weeks. This system enables the generation of fully characterized and banked iPS cell lines ready to use in as little as 9 weeks


Highly efficient and Safe, virus-free reprogramming

The Stemgent mRNA Reprogramming System provides efficiencies greater than 1% as compared to other methods, which yield reprogramming efficiencies varying from 0.00001 to 0.01%. In addition to increased yield of colonies and fast reprogramming kinetics, mRNA reprogramming does not require laborious multi-step passaging or screening for viral or genomic integration once the new colonies are derived.
The mRNA Reprogramming System eliminates virus bio-containment and safety issues, and carries no risk for insertional mutagenesis, an inherent concern with DNA-based reprogramming methods.

Product list

Catalog No. Product Quantity Price
00-0071

mRNA Reprogramming Kit

Content
  • Stemgent mRNA Reprogramming Factors Set: hOKSML
  • Stemgent Pluriton™ Reprogramming Medium
  • B18R Recombinant Protein
- Ask
00-0073

microRNA Booster Kit

Content
  • microRNA Reprogramming Cocktail
  • B18R Recombinant Protein
- Ask
00-0069

Stemgent Stemfect RNA™ Transfection Kit

Content
  • Stemfect™ RNA Transfection Reagent
  • Stemfect™ Buffer
- Ask
01-0005 NutriStemR XF/FF 500 mL Ask
RCHEOT002 bFGF 25 μg Ask
GSC-3002G Nuff cell 4-5×106 cells Ask
GSC-3006G
09-0068 StainAlive™ TRA-1-60 Antibody (DyLight™ 488), Mouse anti-Human 100 μL Ask
09-0069 StainAlive™ TRA-1-81 Antibody (DyLight™ 488), Mouse anti-Human 100 μL Ask
*All of above reagents are necessary for enhanced mRNA reprogramming

Presentations

  • Mandal PK, Rossi DJ. (2013) Reprogramming human fibroblasts to pluripotency using modified mRNA. Nature Protocols. 8(3):568-82
  • Angel M, Yanik MF. (2010) Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins. PLoS One. 5(7):e11756
  • Warren L, et al. (2010) Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell. 5;7(5):618-30.
  • Yakubov E, et al. (2010) Reprogramming of human fibroblasts to pluripotent stem cells using mRNA of four transcription factors. Biochem Biophys Res Commun. 394(1):189-93.