Anti Mouse Nanog Antibody

Product Classification : Pluripotency Marker / AntibodiesResearch Theme : ES/iPS cells

Anti Mouse Nanog Antibody
  • Usable as a marker for detecting ES cells and iPS cells
  • Developed by Prof. Norio Nakatsuji, Kyoto University Institute for Integrated Cell Material Sciences (iCeMS) founding director and Assoc. Prof. Takashi Tada, Kyoto University Institute for Frontier Medical Sciences
  • Widely used in a number of published studies
Store at – 20°C


Transcriptional factors, OCT3/4 (POU5F1) and STAT3 function as key regulators in maintaining pluripotency of stem cells. Thus, POU5F1 and STAT3 have been widely used as molecular markers of pluripotential stem cells. Pluripotential cell-specific Nanog gene is a newly identified homeodomain-bearing transcriptional factor. Importantly, Nanog is expressed specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Therefore, Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human.

ImmunogenMouse Nanog protein
Raised inRabbit
PurityImmunogen affinity purified
Concentration0.20 mg/mL
Tested applicationsWestern blot
Application notesWB: Use at a dilution of 1/300 ? 1/2000
Immunocytochemistry: Use at a dilution of 1/150 ? 1/700
Storage bufferPhosphate buffered saline incl. 0.1% sodium azide
StorageStore at +4°C for short term use. Aliquot and store at -20°C or -80°C.
Avoid repeated freeze / thaw cycles.


IHC using mNanog antibody

IHC using mNanog antibody
Cells: Embryonic Stem Cells (ESCs): HM1-30

Western blot using mNanog antibody

Western blot using mNanog antibody
Embryonic Stem Cells (ESCs): HM1-29


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