Anti Mouse Nanog Antibody
Product Classification : Pluripotency Marker / AntibodiesResearch Theme : ES/iPS cells
- Usable as a marker for detecting ES cells and iPS cells
- Developed by Prof. Norio Nakatsuji, Kyoto University Institute for Integrated Cell Material Sciences (iCeMS) founding director and Assoc. Prof. Takashi Tada, Kyoto University Institute for Frontier Medical Sciences
- Widely used in a number of published studies
Transcriptional factors, OCT3/4 (POU5F1) and STAT3 function as key regulators in maintaining pluripotency of stem cells. Thus, POU5F1 and STAT3 have been widely used as molecular markers of pluripotential stem cells. Pluripotential cell-specific Nanog gene is a newly identified homeodomain-bearing transcriptional factor. Importantly, Nanog is expressed specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Therefore, Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human.
|Immunogen||Mouse Nanog protein|
|Purity||Immunogen affinity purified|
|Tested applications||Western blot
|Application notes||WB: Use at a dilution of 1/300 ? 1/2000
Immunocytochemistry: Use at a dilution of 1/150 ? 1/700
|Storage buffer||Phosphate buffered saline incl. 0.1% sodium azide|
|Storage||Store at +4°C for short term use. Aliquot and store at -20°C or -80°C.
Avoid repeated freeze / thaw cycles.
IHC using mNanog antibody
Cells: Embryonic Stem Cells (ESCs): HM1-30
Western blot using mNanog antibody
Embryonic Stem Cells (ESCs): HM1-29
Safety Data Sheet
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|RCAB001P||Anti mouse Nanog antibody（200μL）||Ask|
|RCAB002P-F||Anti mouse Nanog antibody（100μL）||Ask|
Feeder-Dependent Culture Reagents
|RCHEMD001||Primate ES cell medium (500mL)||Ask|
|RCHEMD001A||Primate ES cell medium (500mL) x5||Ask|
|RCHEMD001B||Primate ES cell medium (500mL) x5 & bFGF(25ug) pack||Ask|
ReproStem（500mL）x5 & bFGF（25ug）pack
|RCHECK002||Primate ES cell culture kit||Ask|
Feeder-Free Culture Reagents
|RCHEMD006B||ReproFF2（500mL）x3 & bFGF（25μL）pack||￥90,400|
|RCHEMD004B||ReproFF（500mL）x5 & bFGF（25μL）pack||Ask|
Othe Culture Reagents
|RCHETP002||Dissociation solution for human ES/iPS cells（30mL）||Ask|
|RCHEFM001||Freezing medium for human ES/iPS cells（25mL）||Ask|