ReproHSC™ (Japan only)

Product Classification : Primary Culture MediaResearch Theme : Hematopoietic Stem Cells

ReproHSC™ (Japan only)

Medium for human hematopoietic stem cell (HSC) culture

  • Highly efficient human hematopoietic stem cell (CD34+CD38-) culture medium
  • A small-molecule agonist of Thrombopoietin (TPO) receptor included
  • Lower culture cost for human HSC, compared to other media
  • Serum-free

Store at – 20°C

Data

Total cell number increased about 40-fold

Total cell number increased about 40-fold

HSCs culture

  • Cell : human cord blood 34+ cells
  • Culture conditions : ReproHSC™ + Stem Cell Factor
  • Term : one week

Hematopoietic stem cells are expanded 20-fold

Hematopoietic stem cells are expanded 20-fold

HSCs (CD34+CD38- cells) were
selectively expanded compared
with Product A after 1 week.

Repopulating cells were increased about 2-fold

Repopulating cells were increased about 2-fold

Transplantation into NOD/SCID mice

  • Cell : human cord blood 34+ cells
  • Culture conditions : ReproHSC™ + Stem Cell Factor + flt3 ligand
  • Term before transplantation: 14 days
  • Term after transplantation: 56 days
  • Analysis : human CD45+ cells in bone marrow

(All data were provided by Dr. A. Iwama at Chiba university)

Reference: Taito Nishino, et al. Ex vivo expansion of human hematopoietic stem cells by a small-molecule agonist of c-MPL. Experimental Hematology 2009;37:1364?1377.

Reference

Reference: Taito Nishino, et al. Ex vivo expansion of human hematopoietic stem cells by a small-molecule agonist of c-MPL. Experimental Hematology 2009;37:1364?1377.

Product List

Catalog No. Product Price
RCHSMD001 ReproHSC™ Ask