Primate ES Cell Medium
Product Classification : hES/iPSC Culture Media / ReagentsResearch Theme : ES/iPS cells
The gold standard culture medium for feeder-dependent culture of hESCs or hiPSCs
- Jointly developed by ReproCELL and Kyoto University
- Just add bFGF before use*. No other mixing required.
- Each lot is functionally tested by using human iPS cells.
- Lot-to-lot control of other critical criteria including osmolarity, pH, sterility and mycoplasma.
Note 1: bFGF is not included in Primate ES Cell Medium. Please purchase bFGF separately and add it before you culture human ES cells and iPS cells.
ReproCELL's Primate ES Cell Medium is designed for feeder-dependent ES (embryonic stem) cell or iPS (induced pluripotent stem) cell culture. Since Shinya Yamanaka, Director and Professor of Kyoto University's Center for iPS Cell Research and Application (CiRA), discovered human iPS cells using Primate ES Cell Medium in 2007, Primate ES cell medium users drastically increased.
Today, Primate ES Cell Medium is widely used in laboratories throughout the world as the most reliable and best suited medium for feeder dependent ES, iPS and other primate cell culture. Primate ES Cell Medium was developed by Kyoto University Prof. Norio Nakatsuji and Assoc. Prof. Hirofumi Suemori in order to simplify the process of human ES and iPS cell culture. In fact preparation is dramatically simplified. All you have to do is to add bFGF prior to usage* and the medium is ready to use.
Please see Primate ES cell related products at the bottom of this page.
*: not required for all cell lines
human iPSC cultured using Primate ES Cell Medium with 5ng/ml bFGF on MEF
[Movie] human iPSC growing in Primate ES Cell Medium
- Lot: B01PL15 Lot: R1M160526
- Lot: R1M160414
- Lot: R1M160309
- Lot: R1M160212
- Lot: R1M160119
- Lot: R1M151215
- Lot: R1M151009
- Lot: R1M150917
- Lot: R1M150820
- Lot: R1M150717
- Lot: R1M150618
- Lot: R1M150430
- Lot: R1M150213
- Lot: R1M141121
- Lot: R1M141107
- Lot: R1M141023
- Lot: R1M141009
- Lot: R1M140919
- Lot: R1M140829
- Lot: R1M140808
- Lot: R1M140711
- Lot: R1M140613
- Lot: R1M140417
- Lot: R1M140220
- Lot: R1M140207
- Lot: R1M140123
- Lot: R1M140110
Safety Data Sheet
The culture procedures for human iPS cells
Instruction movies for human iPS cell culture procedure with ReproCELL's culture reagents.
Chapter 1: Human iPSC colony morphology
Morphology of human iPS cell colonies is important to judge the condition of the culture. Colonies in a good condition show high cell density inside.
Chapter 2: Seeding feeder cells
The plating density of feeder cells is important for human iPS cell culture.
Chapter 3: Passaging human iPS cells
Dissociation Solution for human ES/iPS Cells (CTK solution) makes it possible to passage human iPS cells very easily and successfully with excellent cell viability. Colonies can be divided to appropriate size for passaging just by incubation and pipetting.
Chapter 4: Freezing and thawing human iPS cells
It is possible to cryopreserve human iPS cells at high cell viability by using Freezing Medium for human ES/iPS Cells. This method ulitizes vitrification which minimizes the damage to the cryopreserved cells. It is important to freeze and thaw the cells quickly.
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- D'Souza SS, Maufort J, Kumar A, Zhang J, Smuga-Otto K, Thomson JA, Slukvin II. GSK3β Inhibition Promotes Efficient Myeloid and Lymphoid Hematopoiesis from Non-human Primate-Induced Pluripotent Stem Cells. Stem Cell Reports. 2016 Feb 9;6(2):243-56. doi: 10.1016/j.stemcr.2015.12.010. Epub 2016 Jan 21. PubMed PMID: 26805448.
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- Fujikura, J., et al. “Induced pluripotent stem cells generated from diabetic patients with mitochondrial DNA A3243G mutation.” Diabetologia 55.6 (2012): 1689-1698.
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⇒ Efficient Embryoid Body Formation from Human iPS Cells on Novel Microfabric Vessels
( ISSCR 2015（International Society for Stem Cell Research））（sep. 2015）
Feeder-Dependent Culture Reagents
|RCHEMD001||Primate ES cell medium (500mL)||Ask|
|RCHEMD001A||Primate ES cell medium (500mL) x5||Ask|
|RCHEMD001B||Primate ES cell medium (500mL) x5 & bFGF(25μL) pack||Ask|
Feeder-Dependent Culture Reagents
|RCHEMD005A||ReproStem (500mL) x5||Ask|
|RCHEMD005B||ReproStem (500mL) x5 & bFGF(25μL) pack||Ask|
|RCHECK002||Primate ES cell culture kit||Ask|
Feeder-Free Culture Reagents
|RCHEMD006A||ReproFF2 (500mL) x3||Ask|
|RCHEMD006B||ReproFF2 (500mL) x3 & bFGF(25μL) pack||Ask|
|RCHEMD004A||ReproFF (500mL) x5||Ask|
|RCHEMD004B||ReproFF (500mL) x5 & bFGF(25μL) pack||Ask|
Othe Culture Reagents
|RCHETP002||Dissociation solution for human ES/iPS cells (30mL)||Ask|
|RCHEFM001||Freezing medium for human ES/iPS cells (25mL)||Ask|