Primate ES Cell Medium

Product Classification : hES/iPSC Culture Media / ReagentsResearch Theme : ES/iPS cells

Primate ES Cell Medium

The gold standard culture medium for feeder-dependent culture of hESCs or hiPSCs

  • Jointly developed by ReproCELL and Kyoto University
  • Just add bFGF before use*. No other mixing required.
  • Each lot is functionally tested by using human iPS cells.
  • Lot-to-lot control of other critical criteria including osmolarity, pH, sterility and mycoplasma.
  • Serum-free

Note 1: bFGF is not included in Primate ES Cell Medium. Please purchase bFGF separately and add it before you culture human ES cells and iPS cells.


ReproCELL's Primate ES Cell Medium is designed for feeder-dependent ES (embryonic stem) cell or iPS (induced pluripotent stem) cell culture. Since Shinya Yamanaka, Director and Professor of Kyoto University's Center for iPS Cell Research and Application (CiRA), discovered human iPS cells using Primate ES Cell Medium in 2007, Primate ES cell medium users drastically increased.

Today, Primate ES Cell Medium is widely used in laboratories throughout the world as the most reliable and best suited medium for feeder dependent ES, iPS and other primate cell culture. Primate ES Cell Medium was developed by Kyoto University Prof. Norio Nakatsuji and Assoc. Prof. Hirofumi Suemori in order to simplify the process of human ES and iPS cell culture. In fact preparation is dramatically simplified. All you have to do is to add bFGF prior to usage* and the medium is ready to use.

Please see Primate ES cell related products at the bottom of this page.

*: not required for all cell lines

human iPSC cultured using Primate ES Cell Medium with 5ng/ml bFGF on MEF

[Movie] human iPSC growing in Primate ES Cell Medium


Data Sheet

Safety Data Sheet

Instruction Movies

The culture procedures for human iPS cells

Instruction movies for human iPS cell culture procedure with ReproCELL's culture reagents.

Chapter 1: Human iPSC colony morphology

Morphology of human iPS cell colonies is important to judge the condition of the culture. Colonies in a good condition show high cell density inside.

Chapter 2: Seeding feeder cells

The plating density of feeder cells is important for human iPS cell culture.

Chapter 3: Passaging human iPS cells

Dissociation Solution for human ES/iPS Cells (CTK solution) makes it possible to passage human iPS cells very easily and successfully with excellent cell viability. Colonies can be divided to appropriate size for passaging just by incubation and pipetting.

Chapter 4: Freezing and thawing human iPS cells

It is possible to cryopreserve human iPS cells at high cell viability by using Freezing Medium for human ES/iPS Cells. This method ulitizes vitrification which minimizes the damage to the cryopreserved cells. It is important to freeze and thaw the cells quickly.


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  ⇒ Efficient Embryoid Body Formation from Human iPS Cells on Novel Microfabric Vessels
  ( ISSCR 2015(International Society for Stem Cell Research))(sep. 2015)

Product list

Feeder-Dependent Culture Reagents

Catalog No. Product Price
RCHEMD001 Primate ES cell medium (500mL) Ask
RCHEMD001A Primate ES cell medium (500mL) x5 Ask
RCHEMD001B Primate ES cell medium (500mL) x5 & bFGF(25μL) pack Ask

Related Products

Feeder-Dependent Culture Reagents

Catalog No. Product Price
RCHEMD005 ReproStem (500mL) Ask
RCHEMD005A ReproStem (500mL) x5 Ask
RCHEMD005B ReproStem (500mL) x5 & bFGF(25μL) pack Ask
RCHEOT001 ReproCoat (500mL) Ask
RCHECK002 Primate ES cell culture kit Ask

Feeder-Free Culture Reagents

Catalog No. Product Price
RCHEMD006 ReproFF2 (500mL) Ask
RCHEMD006A ReproFF2 (500mL) x3 Ask
RCHEMD006B ReproFF2 (500mL) x3 & bFGF(25μL) pack Ask
RCHEMD007 ReproXF (500mL) Ask
RCHEMD004 ReproFF (500mL) Ask
RCHEMD004A ReproFF (500mL) x5 Ask
RCHEMD004B ReproFF (500mL) x5 & bFGF(25μL) pack Ask

Growth Factor

Catalog No. Product Price
RCHEOT002 bFGF (25μL) Ask
RCHEOT003 bFGF (250μL) Ask

Othe Culture Reagents

Catalog No. Product Price
RCHETP002 Dissociation solution for human ES/iPS cells (30mL) Ask
RCHEFM001 Freezing medium for human ES/iPS cells (25mL) Ask

Antibodies for Pluripotency Markers

Catalog No. Product Price
RCAB001P Anti mouse Nanog antibody (200μL) Ask
RCAB002P-F Anti mouse Nanog antibody (100μL) Ask
RCAB003P Anti human Nanog antibody (200μL) Ask
RCAB004P-F Anti human Nanog antibody (100μL) Ask