Primate ES Cell Medium

Product Classification : hES/iPSC Culture Media / ReagentsResearch Theme : ES/iPS cells

Primate ES Cell Medium

The gold standard culture medium for feeder-dependent culture of hESCs or hiPSCs

  • Jointly developed by ReproCELL and Kyoto University
  • Just add bFGF before use*. No other mixing required.
  • Each lot is functionally tested by using human iPS cells.
  • Lot-to-lot control of other critical criteria including osmolarity, pH, sterility and mycoplasma.
  • Serum-free

Note 1: bFGF is not included in Primate ES Cell Medium. Please purchase bFGF separately and add it before you culture human ES cells and iPS cells.

Overview

ReproCELL's Primate ES Cell Medium is designed for feeder-dependent ES (embryonic stem) cell or iPS (induced pluripotent stem) cell culture. Since Shinya Yamanaka, Director and Professor of Kyoto University's Center for iPS Cell Research and Application (CiRA), discovered human iPS cells using Primate ES Cell Medium in 2007, Primate ES cell medium users drastically increased.

Today, Primate ES Cell Medium is widely used in laboratories throughout the world as the most reliable and best suited medium for feeder dependent ES, iPS and other primate cell culture. Primate ES Cell Medium was developed by Kyoto University Prof. Norio Nakatsuji and Assoc. Prof. Hirofumi Suemori in order to simplify the process of human ES and iPS cell culture. In fact preparation is dramatically simplified. All you have to do is to add bFGF prior to usage* and the medium is ready to use.

Please see Primate ES cell related products at the bottom of this page.

*: not required for all cell lines

human iPSC cultured using Primate ES Cell Medium with 5ng/ml bFGF on MEF

[Movie] human iPSC growing in Primate ES Cell Medium

Protocol

Data Sheet

Safety Data Sheet

Instruction Movies

The culture procedures for human iPS cells

Instruction movies for human iPS cell culture procedure with ReproCELL's culture reagents.

Chapter 1: Human iPSC colony morphology

Morphology of human iPS cell colonies is important to judge the condition of the culture. Colonies in a good condition show high cell density inside.

Chapter 2: Seeding feeder cells

The plating density of feeder cells is important for human iPS cell culture.

Chapter 3: Passaging human iPS cells

Dissociation Solution for human ES/iPS Cells (CTK solution) makes it possible to passage human iPS cells very easily and successfully with excellent cell viability. Colonies can be divided to appropriate size for passaging just by incubation and pipetting.

Chapter 4: Freezing and thawing human iPS cells

It is possible to cryopreserve human iPS cells at high cell viability by using Freezing Medium for human ES/iPS Cells. This method ulitizes vitrification which minimizes the damage to the cryopreserved cells. It is important to freeze and thaw the cells quickly.

Publications

  • Fukusumi H, Shofuda T, Bamba Y, Yamamoto A, Kanematsu D, Handa Y, Okita K,Nakamura M, Yamanaka S, Okano H, Kanemura Y. Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins. Stem Cells Int. 2016;2016:7235757. doi: 10.1155/2016/7235757. Epub 2016 Apr 26. PubMed PMID: 27212953; PubMed Central PMCID: PMC4861799.
  • D'Souza SS, Maufort J, Kumar A, Zhang J, Smuga-Otto K, Thomson JA, Slukvin II. GSK3β Inhibition Promotes Efficient Myeloid and Lymphoid Hematopoiesis from Non-human Primate-Induced Pluripotent Stem Cells. Stem Cell Reports. 2016 Feb 9;6(2):243-56. doi: 10.1016/j.stemcr.2015.12.010. Epub 2016 Jan 21. PubMed PMID: 26805448.
  • Araoka, Toshikazu, et al. “Efficient and Rapid Induction of Human iPSCs/ESCs into Nephrogenic Intermediate Mesoderm Using Small Molecule-Based Differentiation Methods.” PloS one 9.1 (2014): e84881.
  • Diederichs, Solvig, and Rocky S. Tuan. “Functional Comparison of Human Induced Pluripotent Stem Cell-Derived Mesenchymal Cells and Bone Marrow-Derived Mesenchymal Stromal Cells from the Same Donor.” Stem cells and development ja (2014).
  • Hirata, Nao, et al. “A Chemical Probe that Labels Human Pluripotent Stem Cells.” Cell reports 6.6 (2014): 1165-1174.
  • Kanemura, Hoshimi, et al. “Tumorigenicity Studies of Induced Pluripotent Stem Cell (iPSC)-Derived Retinal Pigment Epithelium (RPE) for the Treatment of Age-Related Macular Degeneration.” PloS one 9.1 (2014): e85336.
  • Kimbrel, Erin A., et al. “Mesenchymal stem cell population derived from human pluripotent stem cells displays potent immunomodulatory and therapeutic properties.” Stem cells and development ja (2014).
  • Koh, Sehwon, and Jorge A. Piedrahita. “From “ES-like” cells to induced pluripotent stem cells: A historical perspective in domestic animals.” Theriogenology 81.1 (2014): 103-111.
  • Morishima, Tatsuya, et al. “Genetic correction of HAX1 in induced pluripotent stem cells from a patient with severe congenital neutropenia improves defective granulopoiesis.” Haematologica 99.1 (2014): 19-27.
  • Takahashi, Daisuke, et al. “Electroporation of Adherent Cells by Direct Lamination of Hydrogel-Based Microelectrode Substrates.” Chemistry Letters 43.4 (2014): 444-446.
  • Fukusumi, Hayato, et al. “Feeder-free generation and long-term culture of human induced pluripotent stem cells using pericellular matrix of decidua derived mesenchymal cells.” PloS one 8.1 (2013): e55226.
  • Haraguchi, Yuji, et al. “Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering.” Journal of tissue engineering and regenerative medicine (2013).
  • Hayakawa, Kazuo, et al. “Identification of target genes of synovial sarcoma-associated fusion oncoprotein using human pluripotent stem cells.” Biochemical and biophysical research communications 432.4 (2013): 713-719.
  • Hitomi, Toshiaki, et al. “Downregulation of Securin by the variant RNF213 R4810K (rs112735431, G> A) reduces angiogenic activity of induced pluripotent stem cell-derived vascular endothelial cells from moyamoya patients.” Biochemical and biophysical research communications 438.1 (2013): 13-19.
  • Hongisto, Heidi. “Fibroblast feeder cells in human pluripotent stem cell culture and retinal differentiation-progress toward clinical cell therapy.” (2013).
  • Horii, Takuro, et al. “Generation of an ICF syndrome model by efficient genome editing of human induced pluripotent stem cells using the CRISPR system.” International journal of molecular sciences 14.10 (2013): 19774-19781.
  • Iida, K., et al. “hypoxia-enhanced Derivation of ipscs from human Dental pulp cells.” Journal of dental research 92.10 (2013): 905-910.
  • Lan, Chen-Wei, et al. “Differentiation of human embryonic stem cells into functional ovarian granulosa-like cells.” The Journal of Clinical Endocrinology & Metabolism 98.9 (2013): 3713-3723.
  • Maeda, Tadao, et al. “Retinal pigmented epithelial cells obtained from human induced pluripotent stem cells possess functional visual cycle enzymes in vitro and in vivo.” Journal of Biological Chemistry 288.48 (2013): 34484-34493.
  • Matsumoto, Yoshihisa, et al. “Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation.” Orphanet journal of rare diseases 8.1 (2013): 190.
  • Nasu, Akira, et al. “Genetically matched human iPS cells reveal that propensity for cartilage and bone differentiation differs with clones, not cell type of origin.” PloS one 8.1 (2013): e53771.
  • Noguchi, Michio, et al. “In vitro characterization and engraftment of adipocytes derived from human induced pluripotent stem cells and embryonic stem cells.” Stem cells and development 22.21 (2013): 2895-2905.
  • Okita, Keisuke, et al. “An Efficient Nonviral Method to Generate Integration‐Free Human‐Induced Pluripotent Stem Cells from Cord Blood and Peripheral Blood Cells.” Stem Cells 31.3 (2013): 458-466.
  • Sakuma, Tetsushi, et al. “Efficient TALEN construction and evaluation methods for human cell and animal applications.” Genes to Cells 18.4 (2013): 315-326.(PrimateかStemかは不明)
  • Takase, Osamu, et al. “The Role of NF-κB Signaling in the Maintenance of Pluripotency of Human Induced Pluripotent Stem Cells.” PloS one 8.2 (2013): e56399.
  • Tanabe, Koji, et al. “INAUGURAL ARTICLE by a Recently Elected Academy Member: Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts.” Proceedings of the National Academy of Sciences of the United States of America 110.30 (2013): 12172.
  • Tanabe, Koji, et al. “Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts.” Proceedings of the National Academy of Sciences 110.30 (2013): 12172-12179.
  • Tanaka, Akihito, et al. “Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro.” PloS one 8.4 (2013): e61540.
  • Fujikura, J., et al. “Induced pluripotent stem cells generated from diabetic patients with mitochondrial DNA A3243G mutation.” Diabetologia 55.6 (2012): 1689-1698.
  • Jung, Dongju, et al. “Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells.” Journal of nanobiotechnology 10.1 (2012): 1-10.
  • Kajiwara, Masatoshi, et al. “Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells.” Proceedings of the National Academy of Sciences 109.31 (2012): 12538-12543.
  • Kunisada, Yuya, et al. “Small molecules induce efficient differentiation into insulin-producing cells from human induced pluripotent stem cells.” Stem cell research 8.2 (2012): 274-284.
  • Kuroda, Takuya, et al. “Highly sensitive in vitro methods for detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human iPS cells.” PloS one 7.5 (2012): e37342.
  • Matsuura, Katsuhisa, et al. “Creation of human cardiac cell sheets using pluripotent stem cells.” Biochemical and biophysical research communications 425.2 (2012): 321-327.
  • Nakahata, Tatsutoshi, et al. “Method for producing dendritic cells from pluripotent stem cells.” U.S. Patent Application 14/001,004.
  • Nakamura, Naoko, et al. “Feeder-free and serum-free production of hepatocytes, cholangiocytes, and their proliferating progenitors from human pluripotent stem cells: application to liver-specific functional and cytotoxic assays.” Cellular Reprogramming (Formerly” Cloning and Stem Cells”) 14.2 (2012): 171-185.
  • Okahara-Narita, Junko, et al. “Induction of pluripotent stem cells from fetal and adult cynomolgus monkey fibroblasts using four human transcription factors.” Primates 53.2 (2012): 205-213.
  • Shinozawa, Tadahiro, et al. “A novel purification method of murine embryonic stem cell?and human-induced pluripotent stem cell?derived cardiomyocytes by simple manual dissociation.” Journal of biomolecular screening 17.5 (2012): 683-691.
  • Shinozawa, Tadahiro, et al. “Determination of Appropriate Stage of Human-Induced Pluripotent Stem Cell?Derived Cardiomyocytes for Drug Screening and Pharmacological Evaluation In Vitro.” Journal of biomolecular screening 17.9 (2012): 1192-1203.
  • Sonoyama, Takuhiro, et al. “Differentiation of human embryonic stem cells and human induced pluripotent stem cells into steroid-producing cells.” Endocrinology 153.9 (2012): 4336-4345.
  • Tanaka, Takayuki, et al. “Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery.” Blood 120.6 (2012): 1299-1308.
  • Wada, Tamaki, et al. “Amyotrophic lateral sclerosis model derived from human embryonic stem cells overexpressing mutant superoxide dismutase 1.” Stem cells translational medicine 1.5 (2012): 396-402.
  • Wakao, Shohei, et al. “Morphologic and gene expression criteria for identifying human induced pluripotent stem cells.” PloS one 7.12 (2012): e48677.
  • Wutz, Anton. “Epigenetic alterations in human pluripotent stem cells: a tale of two cultures.” Cell stem cell 11.1 (2012): 9-15.
  • Iwabuchi, Kumiko A., et al. “ECAT11/L1td1 Is Enriched in ESCs and Rapidly Activated During iPSCGeneration, but It Is Dispensable for the Maintenance and Induction of Pluripotency.” PloS one 6.5 (2011): e20461.
  • Kobayashi, Hideyuki. “Pluripotent Stem Cells Induced from Testicular Tissue of a Man with Klinefelter Syndrome (47, XXY) by Four Transcription Factors (OCT4, SOX2, KLF4, and C-MYC).” (2011).
  • Maekawa, Momoko, et al. “Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1.” Nature 474.7350 (2011): 225-229.
  • Ogawa, Shin-ichiro, et al. “Induction of oligodendrocyte differentiation from adult human fibroblast-derived induced pluripotent stem cells.” In Vitro Cellular & Developmental Biology-Animal 47.7 (2011): 464-469.
  • Okita, Keisuke, et al. “A more efficient method to generate integration-free human iPS cells.” Nature methods 8.5 (2011): 409-412.
  • Takata, Akemi, et al. “Direct differentiation of hepatic cells from human induced pluripotent stem cells using a limited number of cytokines.” Hepatology international 5.4 (2011): 890-898.
  • Tatsumi, Rie, et al. “Simple and highly efficient method for production of endothelial cells from human embryonic stem cells.” Cell transplantation 20.9 (2011): 1423-1430.
  • Yahata, Naoki, et al. “Anti-Aβ drug screening platform using human iPS cell-derived neurons for the treatment of Alzheimer’s disease.” PLoS One 6.9 (2011): e25788.
  • Fujioka, Tsuyoshi, et al. “Establishment of induced pluripotent stem cells from human neonatal tissues.” Human cell 23.3 (2010): 113-118.
  • Kazuki, Yasuhiro, et al. “Complete genetic correction of ips cells from Duchenne muscular dystrophy.” Molecular Therapy 18.2 (2010): 386-393.
  • Lee, Tae-Hee, et al. “Functional recapitulation of smooth muscle cells via induced pluripotent stem cells from human aortic smooth muscle cells.” Circulation research 106.1 (2010): 120-128.
  • Miyoshi, Keiko, et al. “Generation of human induced pluripotent stem cells from oral mucosa.” Journal of bioscience and bioengineering 110.3 (2010): 345-350.
  • Otsuji, Tomomi G., et al. “Progressive maturation in contracting cardiomyocytes derived from human embryonic stem cells: Qualitative effects on electrophysiological responses to drugs.” Stem cell research 4.3 (2010): 201-213.
  • Sakurai, Kenji, et al. “Efficient integration of transgenes into a defined locus in human embryonic stem cells.” Nucleic acids research 38.7 (2010): e96-e96.
  • Tamaoki, N, et al. “Dental pulp cells for induced pluripotent stem cell banking.” Journal of dental research 89.8 (2010): 773-778.
  • Oka, Y., et al. “293FT cells transduced with four transcription factors (OCT4, SOX2, NANOG, and LIN28) generate aberrant ES-like cells.” J Stem cell Regenerative Med 3 (2010): 149-56.
  • Tamaoki, N., et al. “Dental pulp cells for induced pluripotent stem cell banking.”Journal of dental research 89.8 (2010): 773-778.
  • Chen, Hsin-Fu, et al. “A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals.” Human Reproduction 24.1 (2009): 71-80.
  • Hong, Hyenjong, et al. “Suppression of induced pluripotent stem cell generation by the p53?p21 pathway.” Nature 460.7259 (2009): 1132-1135.
  • Nakahara, Masako, et al. “High-efficiency production of subculturable vascular endothelial cells from feeder-free human embryonic stem cells without cell-sorting technique.” Cloning and stem cells 11.4 (2009): 509-522.
  • Ohnuki, Mari, Kazutoshi Takahashi, and Shinya Yamanaka. “Generation and characterization of human induced pluripotent stem cells.” Current protocols in stem cell biology (2009): 4A-2.
  • Wada, Tamaki, et al. “Highly efficient differentiation and enrichment of spinal motor neurons derived from human and monkey embryonic stem cells.” PloS one 4.8 (2009): e6722.
  • Fusaki, Noemi, et al. “Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome.” Proceedings of the Japan Academy. Series B, Physical and biological sciences 85.8 (2008): 348-362.
  • Takahashi, Kazutoshi, et al. “Induction of pluripotent stem cells from adult human fibroblasts by defined factors.” cell 131.5 (2007): 861-872.

Applications

  ⇒ Efficient Embryoid Body Formation from Human iPS Cells on Novel Microfabric Vessels
  ( ISSCR 2015(International Society for Stem Cell Research))(sep. 2015)

Product list

Feeder-Dependent Culture Reagents

Catalog No. Product Price
RCHEMD001 Primate ES cell medium (500mL) Ask
RCHEMD001A Primate ES cell medium (500mL) x5 Ask
RCHEMD001B Primate ES cell medium (500mL) x5 & bFGF(25μL) pack Ask

Related Products

Feeder-Dependent Culture Reagents

Catalog No. Product Price
RCHEMD005 ReproStem (500mL) Ask
RCHEMD005A ReproStem (500mL) x5 Ask
RCHEMD005B ReproStem (500mL) x5 & bFGF(25μL) pack Ask
RCHEOT001 ReproCoat (500mL) Ask
RCHECK002 Primate ES cell culture kit Ask

Feeder-Free Culture Reagents

Catalog No. Product Price
RCHEMD006 ReproFF2 (500mL) Ask
RCHEMD006A ReproFF2 (500mL) x3 Ask
RCHEMD006B ReproFF2 (500mL) x3 & bFGF(25μL) pack Ask
RCHEMD007 ReproXF (500mL) Ask
RCHEMD004 ReproFF (500mL) Ask
RCHEMD004A ReproFF (500mL) x5 Ask
RCHEMD004B ReproFF (500mL) x5 & bFGF(25μL) pack Ask

Growth Factor

Catalog No. Product Price
RCHEOT002 bFGF (25μL) Ask
RCHEOT003 bFGF (250μL) Ask

Othe Culture Reagents

Catalog No. Product Price
RCHETP002 Dissociation solution for human ES/iPS cells (30mL) Ask
RCHEFM001 Freezing medium for human ES/iPS cells (25mL) Ask

Antibodies for Pluripotency Markers

Catalog No. Product Price
RCAB001P Anti mouse Nanog antibody (200μL) Ask
RCAB002P-F Anti mouse Nanog antibody (100μL) Ask
RCAB003P Anti human Nanog antibody (200μL) Ask
RCAB004P-F Anti human Nanog antibody (100μL) Ask