Freezing Medium for human ES and iPS Cells

Product Classification : hES/iPSC Culture Media / ReagentsResearch Theme : ES/iPS cells

Freezing Medium for human ES and iPS Cells

Freezing Medium for human ES and iPS Cells

  • Increase cell viability up to 10% of input cells (>10-fold higher than DMSO)
  • Rapid freezing by vitrification method using liquid nitrogen
  • The frozen cells can be kept under -135 degC
  • Ideal for serum-free applications
  • Each lot is functionally tested for freezing the cells

Overview

Live colonies recovered from the stock using ReproCELL's Freezing Medium

ReproCeLL’s freezing medium for human ES cell and iPS cells are specifically developed for vitrification method, a rapid-freezing technique, by Prof. Norio Nakatsuji, Kyoto University Institute for Integrated Cell Material Sciences (iCeMS) founding director and Assoc. Prof. Hirofumi Suemori, Kyoto University Institute for Frontier Medical Sciences. The advantage of vitrification technique using liquid nitrogen is its efficiency to ensure high cell viability.

While cell viability rate of slow-freezing technique using DMSO is less than 1%, viability rate of vitrification technique using ReproCELL’s feezing medium for human ES cells and iPS cells is 10%.

Serum-free, Ready-to-use. Store at -80 degC for long term storage.

Data

Figure: Comparison of the survival rate of the cells frozen by using ReproCELL’s freezing solution and 10% DMSO
Figure: Comparison of the survival rate of the cells frozen by using ReproCELL’s freezing solution and 10% DMSO

Protocol

Data Sheet

Data Sheet

Instruction Movies

The culture procedures for human iPS cells

Instruction movies for human iPS cell culture procedure with ReproCELL's culture reagents.

Chapter 1: Human iPSC colony morphology

Morphology of human iPS cell colonies is important to judge the condition of the culture. Colonies in a good condition show high cell density inside.

Chapter 2: Seeding feeder cells

The plating density of feeder cells is important for human iPS cell culture.

Chapter 3: Passaging human iPS cells

Dissociation Solution for human ES/iPS Cells (CTK solution) makes it possible to passage human iPS cells very easily and successfully with excellent cell viability. Colonies can be divided to appropriate size for passaging just by incubation and pipetting.

Chapter 4: Freezing and thawing human iPS cells

It is possible to cryopreserve human iPS cells at high cell viability by using Freezing Medium for human ES/iPS Cells. This method ulitizes vitrification which minimizes the damage to the cryopreserved cells. It is important to freeze and thaw the cells quickly.

Publications

  • Saeki, Kumiko. “Feeder-Free Culture for High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells.” Human Embryonic and Induced Pluripotent Stem Cells. Humana Press, (2012):77-294.
  • Toyoda, Masashi, et al. “Generation of Induced Pluripotent Stem Cells from Human Amnion Cells.” Human Embryonic and Induced Pluripotent Stem Cells. Humana Press, (2012): 249-264.
  • Akasaka, Tsukasa, et al. “Maintenance of hemiround colonies and undifferentiated state of mouse induced pluripotent stem cells on carbon nanotube-coated dishes.” Carbon 49.7 (2011): 2287-2299.
  • Kumazaki, Tsutomu, et al. “Establishment of human induced pluripotent stem cell lines from normal fibroblast TIG-1.” Human cell 24.2 (2011): 96-103.

Product list

Catalog No. Product Price
RCHEFM001 Freezing medium for human ES/iPS cells (25mL) Ask

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