bFGF

Product Classification : hES/iPSC Culture Media / ReagentsResearch Theme : ES/iPS cells

bFGF
  • Human recombinant bFGF (FGF-2 Basic Fibroblast growth factor)
  • Optimized for human ES and iPS cell culture
  • Each lot functionally tested in iPS cell culture
  • Affordable pricing

Overview

bFGF is an essential product when you prepare human ES cell and iPS cell culture medium. The growth factor is necessary for the cells to remain in undifferentiated state. * It is typically used at concentration of 4-5ng/ml for both feeder dependent and feeder free culture. Function tests for pluripotency of stem cells are conducted.

Store at -80 degC for long time storage.

Molecular Mass The 146 amino acid residue recombinant protein has a molecular mass of approximately 16.0K
Concentration 1 mg/mL
Buffer 10 mM Tris-HCL, 1mM DTT, 2M NaCL, pH7.5
Filtered 0.22 μm filter
Purity >97%, as determinded by SDS-PAGE and visualized by CBB stain
Activity The biologocal activity of recombinant human FGF basic was monitored in a mitogenic assay by WST8

Data Sheet

Safety Data Sheet

Instruction Movies

The culture procedures for human iPS cells

Instruction movies for human iPS cell culture procedure with ReproCELL's culture reagents.

Chapter 1: Human iPSC colony morphology

Morphology of human iPS cell colonies is important to judge the condition of the culture. Colonies in a good condition show high cell density inside.

Chapter 2: Seeding feeder cells

The plating density of feeder cells is important for human iPS cell culture.

Chapter 3: Passaging human iPS cells

Dissociation Solution for human ES/iPS Cells (CTK solution) makes it possible to passage human iPS cells very easily and successfully with excellent cell viability. Colonies can be divided to appropriate size for passaging just by incubation and pipetting.

Chapter 4: Freezing and thawing human iPS cells

It is possible to cryopreserve human iPS cells at high cell viability by using Freezing Medium for human ES/iPS Cells. This method ulitizes vitrification which minimizes the damage to the cryopreserved cells. It is important to freeze and thaw the cells quickly.

Publications

  • Hirata, Nao, et al. “A Chemical Probe that Labels Human Pluripotent Stem Cells.” Cell reports 6.6 (2014): 1165-1174.
  • Isono, Kaori, et al. “Generation of familial amyloidotic polyneuropathy-specific induced pluripotent stem cells.” Stem Cell Research (2014).
  • Luo, Lan, et al. “Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.” Scientific reports 4 (2014).
  • Mull, Amber N., Amanda Klar, and Christopher S. Navara. “Differential Localization and High Expression of SURVIVIN Splice Variants in Human Embryonic Stem Cells but not in Differentiated Cells Implicate a Role for SURVIVIN in Pluripotency.” Stem Cell Research (2014).
  • Fukamachi, Hiroshi, et al. “CD49fhigh Cells Retain Sphere-Forming and Tumor-Initiating Activities in Human Gastric Tumors.” PloS one 8.8 (2013): e72438.
  • Haraguchi, Yuji, et al. “Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering.” Journal of tissue engineering and regenerative medicine (2013).
  • Liu, Yang, Shinji Sakai, and Masahito Taya. “Impact of the composition of alginate and gelatin derivatives in bioconjugated hydrogels on the fabrication of cell sheets and spherical tissues with living cell sheaths.” Acta biomaterialia 9.5 (2013): 6616-6623.
  • Murakami, Masashi, et al. “The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential.” Biomaterials 34.36 (2013): 9036-9047.
  • Nakajima-Takagi, Yaeko, et al. “Role of SOX17 in hematopoietic development from human embryonic stem cells.” Blood 121.3 (2013): 447-458.
  • Takase, Osamu, et al. “The Role of NF-κB Signaling in the Maintenance of Pluripotency of Human Induced Pluripotent Stem Cells.” PloS one 8.2 (2013): e56399.
  • Terai, Hideki, et al. “Activation of the FGF2-FGFR1 Autocrine Pathway: A Novel Mechanism of Acquired Resistance to Gefitinib in NSCLC.” Molecular Cancer Research 11.7 (2013): 759-767.
  • Higuchi, Takuma, et al. “High Expression of Nuclear Factor 90 (NF90) Leads to Mitochondrial Degradation in Skeletal and Cardiac Muscles.” PloS one 7.8 (2012): e43340.
  • Koyama, Noriaki, et al. “Human induced pluripotent stem cells differentiated into chondrogenic lineage via generation of mesenchymal progenitor cells.” Stem cells and development 22.1 (2012): 102-113.
  • Ishiwata, Toshiyuki, et al. “Enhanced expression of fibroblast growth factor receptor 2 IIIc promotes human pancreatic cancer cell proliferation.” The American journal of pathology 180.5 (2012): 1928-1941.
  • Kunisada, Yuya, et al. “Small molecules induce efficient differentiation into insulin-producing cells from human induced pluripotent stem cells.” Stem cell research 8.2 (2012): 274-284.
  • Matsuura, Katsuhisa, et al. “Creation of human cardiac cell sheets using pluripotent stem cells.” Biochemical and biophysical research communications 425.2 (2012): 321-327.
  • Nomura, Yoshiaki, et al. “Human periodontal ligament fibroblasts are the optimal cell source for induced pluripotent stem cells.” Histochemistry and cell biology 137.6 (2012): 719-732.
  • Sato, Atsuki, et al. “Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer.” International journal of oncology 41.2 (2012): 441-448.
  • Shimojima, Keiko, et al. “Reduced PLP1 expression in induced pluripotent stem cells derived from a Pelizaeus?Merzbacher disease patient with a partial PLP1 duplication.” Journal of human genetics 57.9 (2012): 580-586.
  • Yabe, Tomio, et al. “A peptide found by phage display discriminates a specific structure of a trisaccharide in heparin.” Journal of Biological Chemistry 286.14 (2011): 12397-12406.
  • YAMAMOTO, TeTSuSHI, TOSHIYuKI ISHIwATA, and ZENYA NAITO. “Morphological and cytoskeletal alterations of nervous system tumor cells with different culturing methods.” International journal of oncology 38 (2011): 1253-1258.

Product List

Catalog No. Product Price
RCHEOT002 bFGF(25ug) Ask
RCHEOT003 bFGF(250ug) Ask

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