Product Classification : hES/iPSC Culture Media / ReagentsResearch Theme : ES/iPS cells

  • Human recombinant bFGF (FGF-2 Basic Fibroblast growth factor)
  • Optimized for human ES and iPS cell culture
  • Each lot functionally tested in iPS cell culture
  • Affordable pricing


bFGF is an essential product when you prepare human ES cell and iPS cell culture medium. The growth factor is necessary for the cells to remain in undifferentiated state. * It is typically used at concentration of 4-5ng/ml for both feeder dependent and feeder free culture. Function tests for pluripotency of stem cells are conducted.

Store at -80 degC for long time storage.

Molecular Mass The 146 amino acid residue recombinant protein has a molecular mass of approximately 16.0K
Concentration 1 mg/mL
Buffer 10 mM Tris-HCL, 1mM DTT, 2M NaCL, pH7.5
Filtered 0.22 μm filter
Purity >97%, as determinded by SDS-PAGE and visualized by CBB stain
Activity The biologocal activity of recombinant human FGF basic was monitored in a mitogenic assay by WST8

Data Sheet

Safety Data Sheet

Instruction Movies

The culture procedures for human iPS cells

Instruction movies for human iPS cell culture procedure with ReproCELL's culture reagents.

Chapter 1: Human iPSC colony morphology

Morphology of human iPS cell colonies is important to judge the condition of the culture. Colonies in a good condition show high cell density inside.

Chapter 2: Seeding feeder cells

The plating density of feeder cells is important for human iPS cell culture.

Chapter 3: Passaging human iPS cells

Dissociation Solution for human ES/iPS Cells (CTK solution) makes it possible to passage human iPS cells very easily and successfully with excellent cell viability. Colonies can be divided to appropriate size for passaging just by incubation and pipetting.

Chapter 4: Freezing and thawing human iPS cells

It is possible to cryopreserve human iPS cells at high cell viability by using Freezing Medium for human ES/iPS Cells. This method ulitizes vitrification which minimizes the damage to the cryopreserved cells. It is important to freeze and thaw the cells quickly.


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Product List

Catalog No. Product Price
RCHEOT002 bFGF(25ug) Ask
RCHEOT003 bFGF(250ug) Ask

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ReproStem(500mL)x5 & bFGF(25ug)pack

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Feeder-Free Culture Reagents

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RCHEMD007 ReproXF(500mL) Ask
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RCHEMD004A ReproFF(500mL) x5 Ask
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Othe Culture Reagents

Catalog No. Product Price
RCHETP002 Dissociation solution for human ES/iPS cells(30mL) Ask
RCHEFM001 Freezing medium for human ES/iPS cells(25mL) Ask

Antibodies for Pluripotency Markers

Catalog No. Product Price
RCAB001P Anti mouse Nanog antibody(200μL) Ask
RCAB002P-F Anti mouse Nanog antibody(100μL) Ask