MEF Feeder Cells

Research Theme : ES/iPS cells

MEF
summary

Features

 ・Mouse Embryonic Fibroblast feeder cells derived from mouse embryo.
 ・A feeder cell line applicable for culture of mouse, primate, human ES/iPS cells.
 ・Validated high quality with every lot.
 ・Mitomycin C-treated cells ready for use.

Storage conditions: liquid nitrogen

This product is very thermosensitive. Upon delivery please move the cell vials into liquid nitrogen to ensure optimal cell attachment levels.


Overview

MEF (Mouse Embryonic Fibroblasts) feeder cells are applicable for a wide range of ES/iPS cell culture. ReproCELL offers exceptionally consistent quality thanks to rigorous validation testing. See protocol (below) for more information.

Figure 1: Undifferentiated ES cells cultured on MEF feeder cells.


Product Info

Catalog No. Product Price
RCHEFC003 MEF(3x106cell)x5 Contact us

3×106cells (1 vial)

References

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  • Kim, Mee‐Hae, Eri Masuda, and Masahiro Kino‐Oka. "Kinetic analysis of deviation from the undifferentiated state in colonies of human induced pluripotent stem cells on feeder layers." Biotechnology and bioengineering (2014).
  • Haraguchi, Yuji, et al. "Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering." Journal of tissue engineering and regenerative medicine (2013).
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  • Chen, Ling, et al. "A model of cancer stem cells derived from mouse induced pluripotent stem cells." PLoS One 7.4 (2012): e33544.
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  • IMAI, Koichi, et al. "An Attempt to Cell Differentiation in Three-dimensional Culture System Using Non-feeder ES-D3 Cells and Feeder Layer Type ES cells." Journal of Oral Tissue Engineering 8.3 (2011): 203-211.
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  • Kobayashi, Hideyuki. "Pluripotent Stem Cells Induced from Testicular Tissue of a Man with Klinefelter Syndrome (47, XXY) by Four Transcription Factors (OCT4, SOX2, KLF4, and C-MYC)." (2011).
  • Kobayashi, Hideyuki, et al. "Reprogramming of adult human testicular cells by four transcription factors (OCT4, SOX2, KLF4, and C-MYC)." Reproductive medicine and biology 10.2 (2011): 105-112.
  • Sakai, Y., and K. Nakazawa. "Novel Microchip Technique for the Transfer of Spheroids as Floating Cultures to Micropatterned-Adherent Cultures." J Biochip Tissue chip S 4 (2011): 2153-0777.
  • Sakai, Yusuke, Yukiko Yoshiura, and Kohji Nakazawa. "Embryoid body culture of mouse embryonic stem cells using microwell and micropatterned chips." Journal of bioscience and bioengineering 111.1 (2011): 85-91.
  • Oka, Y., et al. "293FT cells transduced with four transcription factors (OCT4, SOX2, NANOG, and LIN28) generate aberrant ES-like cells." J Stem cell Regenerative Med 3 (2010): 149-56.
  • Lee, Tae-Hee, et al. "Functional recapitulation of smooth muscle cells via induced pluripotent stem cells from human aortic smooth muscle cells."Circulation research 106.1 (2010): 120-128.
  • Sakai, Yusuke, et al. "Effect of microwell chip structure on cell microsphere production of various animal cells." Journal of bioscience and bioengineering110.2 (2010): 223-229.