ReproHP Medium™

Product Classification : Cell Culture MediumResearch Theme : 3D culture, Hepatitis, Safety Test, Toxicology test

ReproHP Medium
summary

≪A breakthrough in the culture of Primary Human Hepatocytes for enhanced expression of detoxification enzymes.≫

ReproHP Medium™ enables the robust detection of most detoxification enzymes in primary human hepatocytes due to boosted expression levels and lasting CYP activity in culture.


※Storage temperature:-20℃
※Toxicity: contain 0.19% CHIR99021 (in supplement)

≪Higher Cytochrome P450 expression levels.≫

When cultured in ReproHP Medium™ and exposed to specific drugs, the basal and induced expression levels of key detoxification enzymes (CYP3A4, CYP1A2, CYP2B6) are significantly elevated in primary human hepatocytes.

シトクロムP450遺伝子の発現

*Experimental Notes: Reported expression values are normalized against the basal expression level of another “leading brand”. The data shown is from one lot of primary human hepatocytes (Life Technologies) taken at Day 4 in culture. Other human primary hepatocyte lots showed similar patterns of enhanced expression with ReproHP Medium™.

lasting CYP activity

≪3D Culture Leads to Higher Performance≫

Culturing primary human hepatocytes on the Alvetex scaffold in ReproHP Medium™ further boosts CYP3A4 basal expression levels and induced levels after drug induction.

3次元培養による高機能維持

Product List

Catalog No. Product Price
RCRM201 ReproHP Medium™ ASK

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Presentation

・30th JSSX Annual Meeting in Tokyo Poster session November 2015
・International Society for the Study of Xenobiotics (ISSX) (USA) Poster session October 2015


Data Sheet