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Drug Discovery Assay Services Assay: Contractile force in electrically stimulated human stomach (Motilin receptor)

Code: B092
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Assay type:GI Tract
Tissue:Human Stomach (Healthy)
Target:Motilin receptor
Control Compound:Erythromycin
Study Type:EFS organ bath
Functional Endpoint:Contractile force

Assay Description

This assay asseses whether test articles cause a change in contractile force in electrically stimulated human stomach, with erythromycin as a reference compound.

When a bolus of food enters the stomach; its contents and the distention of the gastric wall cause the secretion of various compounds, such as H+, pepsinogen, HCO3 and mucus. These secretions are essential for the initial digestion of proteins and the protection of the stomach mucosa. Movement of the stomach is essential for the mixing of the stomach contents with gastric juices and to move the partially digested food from the stomach into the intestines.

Example assay graph

Image Legend

Figure 1: Cumulative concentration response curve to erythromycin and its vehicle in isolated human stomach, demonstrating a concentration dependent increase in EFS response.

The maximum increase in EFS response was 1752 ± 273.5 % compared to a maximum increase of 95.78 ± 3.22 % for the vehicle.

Testing Information

Introduction

The specific results that will be provided are the effects of increasing concentrations of test articles on the contractile state of isolated human stomach muscle.

Turnaround Time

6 weeks from sample receipt for n = 3 donors.

Timelines are a guide based on Biopta's standard tissue criteria; any client imposed deviations to the standard tissue criteria can alter the project timelines.

Test Article Requirements

Test article(s) to be provided by the Sponsor in storable aliquots at required test concentrations with information on diluent vehicle used. Stock solutions are prepared in distilled water unless otherwise requested. Bath volumes are 25 mL; Sponsor to provide sufficient test article to run the entire study.

Suggested Testing

In duplicate at 6 concentrations.

Study Outline

Rationale and Experimental Design

This assay asseses whether test articles cause a change in contractile force in electrically stimulated human stomach, with erythromycin as a reference compound.

Exclusion Criteria

No specific exclusion criteria are in place other than to reject macroscopically diseased/necrotic tissue. Furthermore, tissues which do not respond to the standard pharmacology checks will be excluded.

Standardisation and Qualification

All individual muscle strips are initially processed through standardisation and qualification procedures to ensure functionality, prior to starting the study protocol.

Muscle strips are processed through a standardisation procedure to reduce signal variability prior to electrical field stimulation and pharmacological intervention. This ensures that muscle strips are maintained under appropriate physiological tension throughout the experiments.

Following standardisation, muscle strips are subjected to an electrical field stimulation (EFS) voltage curve. This process determines the optimal EFS settings to be utilised during the study protocol. Test tissues are then washed and the EFS responses allowed to settle to steady baseline levels.

Muscle strips which pass the standardisation and qualification pass/fail criteria will then progress to the study protocol.

Smooth Muscle Contractility Assay

To assess their ability to modulate EFS mediated smooth muscle contractility, 6 point cumulative concentration response curves will be performed for each test article. These concentration response curves (CCRC's) will be performed upon a steady baseline EFS response. A positive control compound and representative test article vehicle CCRC will also be run to allow direct comparison with test articles.

An example of the conditions assessed for 3 test articles are detailed below (each condition will be run in duplicate muscle strip preparations):

  • Representative test article vehicle CCRC
  • Positive control CCRC
  • Test article 1 CCRC
  • Test article 2 CCRC
  • Test article 3 CCRC

Analysis

Responses shall be expressed as % change from baseline EFS response. Statistical analysis (where appropriate) will be performed using GraphPad Prism, with the results being shown in graphical form in the final report.

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