ReproCryo RM is an optimized freezing medium for ES/iPS cells which does not employ DMSO as a functional additive. DMSO is considered non-desirable due to proven mutagentic and carcinogenic effects. The medium is manufactured under GMP standards from materials that meet the Japan Standards for Biological Materials Safety.
This is the GMP version of ReproCryo DMSO Free (Cat. No. RCHEFM005)
hiPSC 201B7 cell grown in ReproXF medium (Cat. No. RCHEMD007) on vitronectin were cryopreserved with ReproCryo DMSO Free and competitive media, and stored at −80 °C. A vial was thawed and plated to determine viability.
hiPSC 201B7 cells were cryopreserved with ReproCryoDMSO Free, thawed and plated on vitronectin in ReproXF medium (Cat. No. RCHEMD007). After recovery, the cells were stained for Oct3/4 and Nanog. The scale bar is 100 µm
GMP Grade Grade (materials meet Japan Standards for Biological Materials Safety)
Contains no DMSO (dimethyl sulfoxide)
Storage and Stability
Store at −20 °C soon after arrival. Thaw before use, and store at 2 °C to 8 °C after thawing. Use it up within about two weeks after thawing. Avoid repeated freezing and thawing