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Matrixome iMatrix 221 Cardiac and Myoblast Cell Culture Substrate

Code: NP892-06
  • $621.00
  • (Delivery from $65.00)
Available | Usually dispatched within 24 hours
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Recombinant Laminin-221 E-8 Fragments

iMatrix-221's features make it an ideal matrix for some cell culture applications:

  • Xeno-free formulation / CHO-S cell bioproduction
  • Easy to use (liquid format)
  • E8-fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-221

iMatrix-221 is a highly purified and refined product of human recombinant laminin-221 (E8 fragment) expressed by CHO-S cells. Laminin-221 is found predominantly in the basal lamina of striated muscle tissues. Expression deficiencies are implicated in muscular dystophy and cardiomyopathies.1

Laminins of the α-2 type are also a predomiant form found in the human adult heart tissue.2 Cultivation of iPSC-drived cardiomyocytes on iMatrix-221 has been demonstrated to maintain strong contractility in attached monolayer culture.3

Product Name

iMatrix-221 Cardiac and Myoblast Cell Culture Substrate

Catalog Number

NP892-061

NP892-062

Size

  • NP892-061 — 2 × 175 µg
  • NP892-062 — 6 × 175 µg

Molecular Weight

150 kDa

Purity

>95% pure

Formulation

Purified Laminin-221 E8 proteolytic fragment

Storage and Stability

Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.

Quality Control

Activity: Kd for integrin binding

Sterility

Sterile

Notice To Purchaser

REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market.

Recommended Usage

iMatrix-221 is suitable for use as a substrate for culture of various myoblast cell types, but most notably for cardiomyocytes.

Concentration

500 µg/mL

Source

CHO-S cell expression

Manufacturer

Matrixome Corporation (Japan)

Safety Data SHeets

  1. Oliviero P. et.al., Expression of laminin α2 chain during normal and pathological growth of myocardium in rat and human. Cardiovascular Research 46, 346-355 (2000).
  2. Ja K.P. Myu Mia et.al., PSC-derived human cardiac progenitor cells improve ventricular remodeling via angiogenesis and interstitial networking of infarcted myocardium. J Cell Mol Med 20(2), 323-332 (2016).
  3. unpublished, Osaka University(2018).
  4. Miyazaki T. et al. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nature Communications 3: 1236 (2012).
  5. Taniguchi Y. et al., The C-terminal region of laminin β-chains modulates the integrin-binding affinities of laminins. J. Biol. Chem.284 (12): 7820-31 (2009).
  6. Ido H. et al., The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by laminins. J. Biol. Chem. 282 (15): 11144-54 (2017).
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