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Stemgent StemAb TRA-1-81 Antibody (Purified), Mouse anti-Human

Code: 09-0011-base
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The TRA-1-81 monoclonal antibody reacts with a pluripotent-stem-cell-specific antigen expressed upon the surface of human embryonic stem (ES) cells, embyronal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific; as human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost, making it a widely used marker to characterize human ES cells and to monitor their differentiation. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

ICC of H1 cells with TRA-1-81 antibody

ICC analysis of TRA-1-81 on H1 human ES cells at 20x magnification. Cells were stained with TRA-1-81 Antibody (Purified) at a 1:100 dilution followed by a secondary DyLight 488 conjugated antibody (green). DAPI staining was performed to visualize nuclei (blue).

ICC of H1 cells with TRA-1-81 antibody

Immunocytochemistry analysis of TRA-1-81 on H1 human ES cells. Cells were stained with TRA-1-81 Antibody (Purified) at a 1:100 dilution followed by a secondary Cy™ 3 conjugated antibody (red). DAPI staining was performed to visualize nuclei (blue).

Flow cytometry on H1 cells using TRA-1-81 antibody

Flow cytometry of TRA-1-81 on H1 human ES cells. Purple histogram represents TRA-1-81 Antibody (Purified) and open histogram represents isotype control at same concentration.

Product Name

StemAb TRA-1-81 Antibody (Purified), Mouse anti-Human

Catalog Number

09-0011

Size

100 µL

Formulation

Phosphate-buffered solution, pH 7.2, and 0.09% sodium azide

Storage and Stability

Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.

Quality Control

Tested by immunocytochemistry and flow cytometry to ensure product quality.

Recommended Usage

The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and 0.25 µg per 1 × 106 viable cells in 100 µL for flow cytometry. For an application specific protocol, please seeProtocol: ImmunocytochemistryandProtocol: Immunofluorescence Staining of Cells for Flow Cytometry.

Concentration

0.5mg/mL

Isotype

Mouse IgM, κ

Immunogen

Human embryonal carcinoma cell line 2102Ep

Reactivity

Human

Preparation

This antibody was purified by affinity chromatography.

Clone

Tra-1-81

  1. Andrews, P.W., Banting, G., Damjanov, I., Arnaud, D., and Avner, P. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361.
  2. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.
  3. Henderson, J.K., Draper, J.S., Baillie, H.S., Fishel, S., Thomson, J.A., Moore, H., and Andrews, P.W. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337.
  4. Schopperle, W.M., and DeWolf, W.C. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730.

Additional Publications

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