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Stemgent StemAb TRA-1-60 Antibody (Purified), Mouse anti-Human

Code: 09-0010-base
  • $216.00
  • (Delivery from $41.67)
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The TRA-1-60 monoclonal antibody reacts with a pluripotent-stem-cell-specific antigen expressed on undifferentiated human pluripotent stem (PS) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-60 on human PS cells is down-regulated upon differentiation.The TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

 

 

 

Immunocytochemistry analysis of TRA-1-60 on H1 human ES cells. Cells were stained with TRA-1-60 Antibody (Purified) at a 1:100 dilution followed by a secondary Cy 3 antibody (red). DAPI staining was performed to visualize nuclei (blue).

Flow cytometry analysis of TRA-1-60 on H1 human ES cells. Purple histogram represents TRA-1-60 Antibody (Purified) and open histogram represents isotype control at the same concentration.

 

Product Name

StemAb TRA-1-60 Antibody (Purified), Mouse anti-Human

Catalog Number

09-0010

Size

100 µL

Formulation

Phosphate-buffered solution, pH 7.2, and 0.09% sodium azide

Storage and Stability

Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.

Quality Control

Tested by immunocytochemistry and flow cytometry to ensure product quality.

Notice To Purchaser

Purchaser represents and warrants that it will use StemAb TRA-1-60 Antibody (Purified), Mouse anti-Human purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Recommended Usage

The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and 0.25 µg per 1 × 10viable cells in 100 µL for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunofluorescence Staining of Cells for Flow Cytometry.

Concentration

0.5mg/mL

Isotype

Mouse IgM

Immunogen

Human embryonal carcinoma cell line 2102Ep

Reactivity

Human

Preparation

This antibody was purified by affinity chromatography.

Clone

TRA-1-60

  1. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
  2. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.
  3. Schopperle, W.M., and DeWolf, W.C. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730.
  4. Chin, A.C., Fong, W.J., Goh, L.T., Philp, R., Oh, S.K., and Choo, A.B. (2007) Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol. 130: 320-328.
  5. Badcock, G., Pigott, C., Goepel, J., and Andrews, P.W. (1999) The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res. 59: 4715-4719.
  6. Andrews, P.W., Goodfellow, P.N., Shevinsky, L., Bronson, D.L., and Knowles, B.B. (1982) Cell surface antigens of a clonal human embryonic carcinoma cell line: morphological and antigenic differentiation in culture. Int. J. Cancer 29: 523-531.

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