The SSEA-3 monoclonal antibody reacts with stage-specific embryonic antigen-3 (SSEA-3), a carbohydrate epitope on the major ganglioside that is expressed upon the surface of human embryonal carcinoma (EC) cells, embryonic germ (EG) cells, and pluripotent stem (PS) cells. The expression of SSEA-3 on human PS cells is down-regulated upon differentiation. In mouse, SSEA-3 is expressed on unfertilized eggs and preimplantation to early cleaved embryos, and no immunoreactivity is evident in undifferentiated murine EC, ES, and EG cells. SSEA-3 antibody can be used to characterize human ES cells and monitor their differentiation.
Immunocytochemistry analysis of SSEA-3 on H1 human ES cells. Cells were stained with SSEA-3 Antibody (Purified) at a 1:100 dilution followed by a secondary Cy 3 antibody (red). DAPI staining was performed to visualize nuclei (blue).
Flow cytometry analysis of SSEA-3 on H1 human ES cells. Purple histogram represents StemAb SSEA-3 Antibody (Purified) and open histogram represents isotype control at the same concentration.
StemAb SSEA-3 Antibody (Purified), Rat anti-Mouse/Human
Phosphate-buffered solution, pH 7.2, and 0.09% sodium azide
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Tested by immunocytochemistry and flow cytometry to ensure product quality.
Notice To Purchaser
Purchaser represents and warrants that it will use StemAb SSEA-3 Antibody (Purified), Rat anti-Mouse/Human purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and 0.25µg per 1 × 106viable cells in 100µL for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunofluorescence Staining of Cells for Flow Cytometry.
Rat IgM, κ
4-8 cell stage mouse embryos
This antibody was purified by affinity chromatography.