Stage-specific embryonic antigen-1 (SSEA-1), also known as CD15, is an antigenic epitope comprising a Lewis × carbohydrate structure. It is expressed on murine embryonal carcinoma (EC) cells, murine embryonic stem (ES) cells, and murine and human germ cells. SSEA-1 is widely used as a positive surface marker for mouse undifferentiated stem cells and a negative surface marker for human undifferentiated stem cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression. SSEA-1 is associated with cell adhesion, migration, and differentiation. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide. ES cell morphology, proliferation, and expression of pluripotency markers does not change after staining with this antibody.
ICC analysis of SSEA-1 Antibody on R1 mouse ES cells at 10X magnification. [A] Phase contrast view of cells in culture, [B] same field of view after staining with StainAlive SSEA-1 Antibody (DyLight 550) using a 1:100 dilution.
StainAlive SSEA-1 Antibody (DyLight 550), Mouse anti-Mouse/Human
Phosphate-buffered solution, pH 7.2, containing no preservative
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed.
Tested by immunocytochemistry to ensure product quality. Endotoxin level is less than 0.1 EU/µg (0.01 ng/µg) of the protein as determined by the LAL assay.
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. For an application specific protocol, please reference General Protocol for ICC Staining of Live Cells.
Mouse IgM, κ
Mouse F9 teratocarcinoma stem cells (X-irradiated)
This antibody was purified by affinity chromatography and conjugated with DyLight 550 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. The formulation is 0.2 µm filter sterilized and free from bacteria, fungi, mycoplasma, and virus.