Stemgent StainAlive SSEA-1 Antibody (DyLight 488), Mouse anti-Mouse/Human

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Stage-specific embryonic antigen-1 (SSEA-1), also known as CD15, is an antigenic epitope defined as a Lewis × carbohydrate structure. It is expressed on murine embryonal carcinoma (EC) cells, murine embryonic stem (ES) cells, and murine and human germ cells. SSEA-1 is widely used as a positive surface marker for mouse undifferentiated stem cells and a negative surface marker for human undifferentiated stem cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression. SSEA-1 is associated with cell adhesion, migration, and differentiation. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide. ES cell morphology, proliferation, and expression of pluripotency markers do not change after staining with this antibody.

R1 Mouse ESCs stained with 09-0067

Immunocytochemical analysis of SSEA-1 on R1 mouse ES cells. Phase contrast view of cells in culture and the same field of view after staining with StainAlive SSEA-1 Antibody (DyLight 488) using a 1:100 dilution.

Product Name

StainAlive SSEA-1 Antibody (DyLight 488), Mouse anti-Mouse/Human

Catalog Number

09-0067

Size

100 µL

Formulation

Phosphate-buffered solution, pH 7.2, containing no preservative

Storage and Stability

Store at 4 °C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed.

Quality Control

Tested by immunocytochemistry to ensure product quality. Endotoxin level is less than 0.1 EU/µg (0.01 ng/µg) of the protein as determined by the LAL assay.

Recommended Usage

The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. For an application specific protocol, please see 

Concentration

0.5 mg/mL

Isotype

Mouse IgM, κ

Immunogen

Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)

Reactivity

Mouse, Human

Preparation

This antibody was purified by affinity chromatography and conjugated with DyLight 488 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. 0.2 µm filter sterilized.

Formulation 

Free from bacteria, fungi, mycoplasma, and mouse virus.

Clone

MC-480

 
  1. Brambrink, T., Foreman, R., Welstead, G.G., Lengner, C.J., Wernig, M., Suh, H., and Jaenisch, R. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159.
  2. Fox, N., Damjanov, I., Martinez-Hernandez, A., Knowles, B.B., and Solter, D. (1981) Immunohistochemical localization of the early embryonic antigen (SSEA-1) in postimplantation mouse embryos and fetal and adult tissues. Dev Biol 83: 391-398.
  3. Andrews, P.W., Goodfellow, P.N., Shevinsky, L., Bronson, D.L., and Knowles, B.B. (1982) Cell surface antigens of a clonal human embryonic carcinoma cell line: morphological and antigenic differentiation in culture. Int J Cancer 29: 523-531.
  4. Draper, J.S., Pigott, C., Thomson, J.A., and Andrews, P.W. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat 200: 249-258.
  5. Cui, L., Johkura, K., Yue, F., Ogiwara, N., Okouchi, Y., Asanuma, K., and Sasaki, K. (2004) Spatial distribution and initial changes of SSEA-1 and other cell adhesion-related molecules on mouse embryonic stem cells before and during differentiation. J Histochem Cytochem 52: 1447-1457.

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