Stemgent StemAb SSEA-4 Antibody (Purified), Mouse anti-Human

Code: 09-0006-base
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Stage-specific embryonic antigen-4 (SSEA-4) is expressed in a variety of human cell lines including embryonic carcinoma (EC), embryonic stem (ES), and induced pluripotent stem (iPS) cells. SSEA-4 is down-regulated upon differentiation, making it a widely used marker to characterize human ES cells and monitor their differentiation. In mouse, SSEA-4 is expressed on oocytes and early cleavage-stage embryos, but not on EC or ES cells.

 

 

Immunocytochemistry analysis of SSEA-4 on H9 human ES cells. Cells were stained with SSEA-4 Antibody (Purified) at a 1:100 dilution followed by a Cy 3 conjugated secondary antibody (red). DAPI staining was performed to visualize nuclei (blue).

Flow cytometry analysis of SSEA-4 on H9 human ES cells. Purple histogram represents SSEA-4 Antibody (Purified) and open histogram represents isotype control at the same concentration.

Product Name

StemAb SSEA-4 Antibody (Purified), Mouse anti-Human

Catalog Number

09-0006

Size

100 µL

Formulation

Phosphate-buffered solution, pH 7.2, and 0.09% sodium azide

Storage and Stability

Store undiluted at 4 °C and protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.

Quality Control

Tested by immunocytochemistry and flow cytometry to ensure product quality.

Notice To Purchaser

Purchaser represents and warrants that it will use StemAb SSEA-4 Antibody (Purified), Mouse anti-Human purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. REPROCELL will not be held responsible for patent infringement or other violations that may occur with the use of our products

Recommended Usage

The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and 0.25 µg per 1x10viable cells in 100 µL for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunofluorescence Staining of Cells for Flow Cytometry.

Concentration

0.5 mg/mL

Isotype

Mouse IgG3

Immunogen

Human embryonic carcinoma cell line 2102 Ep

Reactivity

Human

Preparation

This antibody was purified by affinity chromatography.

Clone

MC-813-70

  1. Brimble, S.N., Sherrer, E.S., Uhl, E.W., Wang, E., Kelly, S., Merrill, A.H. Jr., Robins, A.J., and Schulz, T.C. (2007) The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Stem Cells 25: 54-62.
  2. Kannagi, R., Cochran, N.A., Ishigami, F., Hakomori, S., Andrews, P.W., Knowles, B.B., and Solter, D. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J 2: 2355-2361.
  3. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
  4. Henderson, J.K., Draper, J.S., Baillie, H.S., Fishel, S., Thomson, J.A., Moore, H., and Andrews, P.W. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337.
  5. Battula, V.L., Bareiss, P.M., Treml, S., Conrad, S., Albert, I., Hojak, S., Abele, H., Schewe, B., Just, L., Skutella, T., and Buhring, H.J. (2007) Human placenta and bone marrow derived MSC cultured in serum-free, b-FGF-containing medium express cell surface frizzled-9 and SSEA-4 and give rise to multilineage differentiation. Differentiation 75: 279-291.

Additional Publications

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