Stage-specific embryonic antigen-4 (SSEA-4) is expressed in a variety of human cell lines including embryonic carcinoma (EC), embryonic stem (ES), and induced pluripotent stem (iPS) cells. SSEA-4 is down-regulated upon differentiation, making it a widely used marker to characterize human ES cells and monitor their differentiation. In mouse, SSEA-4 is expressed on oocytes and early cleavage-stage embryos, but not on EC or ES cells.
Immunocytochemistry analysis of SSEA-4 on H9 human ES cells. Cells were stained with SSEA-4 Antibody (Purified) at a 1:100 dilution followed by a Cy 3 conjugated secondary antibody (red). DAPI staining was performed to visualize nuclei (blue).
Flow cytometry analysis of SSEA-4 on H9 human ES cells. Purple histogram represents SSEA-4 Antibody (Purified) and open histogram represents isotype control at the same concentration.
StemAb SSEA-4 Antibody (Purified), Mouse anti-Human
Phosphate-buffered solution, pH 7.2, and 0.09% sodium azide
Storage and Stability
Store undiluted at 4 °C and protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Tested by immunocytochemistry and flow cytometry to ensure product quality.
Notice To Purchaser
Purchaser represents and warrants that it will use StemAb SSEA-4 Antibody (Purified), Mouse anti-Human purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. REPROCELL will not be held responsible for patent infringement or other violations that may occur with the use of our products
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and 0.25 µg per 1x106 viable cells in 100 µL for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunofluorescence Staining of Cells for Flow Cytometry.
Human embryonic carcinoma cell line 2102 Ep
This antibody was purified by affinity chromatography.