The Goat anti-Rabbit IgG Antibody (DyLight 488) reacts with rabbit IgG and the light chains of other rabbit immunoglobulins. Cross reactivity against non-immunoglobulin serum proteins has not been detected.
Immunocytochemical analysis of Oct4 on H1 human ES cells. Cells were fixed and stained with Oct4 Antibody (Cat. No. 09-0023) using a 1:100 dilution followed by Goat anti-Rabbit IgG Antibody (DyLight 488) (green). DAPI staining was performed to visualize nuclei (blue).
Flow cytometric analysis of Oct4 on H1 human ES cells. Purple histogram represents Oct4 Antibody followed by Goat anti-Rabbit IgG Antibody (DyLight 488) and open histogram represents isotype control at the same concentration.
StemAb Goat anti-Rabbit IgG Antibody (DyLight 488)
Phosphate-buffered solution, pH 7.2, and 0.09% sodium azide
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Tested by immunocytochemistry and flow cytometry to ensure product quality.
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and a 1:20 dilution for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunoflourescence Staining of Cells for Flow Cytometry.
This antibody was purified by affinity chromatography and conjugated with DyLight 488 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody.