Stemgent StemAb Alkaline Phosphatase Staining Kit II

Code: 00-0055-base
  • $97.00
  • (Delivery from $65.00)
Available | Usually dispatched within 24 hours
Prices apply to customers in North America only
Loading...
Be the first to review Stemgent StemAb Alkaline Phosphatase Staining Kit II. Write a review

Alkaline phosphatase (AP) is expressed at high levels in pluripotent stem cells. AP dephosphorylates nucleotides, proteins, and alkaloids under alkaline conditions. After staining, undifferentiated cells appear red or purple whereas differentiated cells appear colorless.

The undifferentiated state of embryonic stem (ES) and induced pluripotent stem (iPS) cells can be characterized by a high level of alkaline phosphatase (AP) expression which, along with the expression of other surface markers, indicates undifferentiated cells with the potential to self-renew. AP is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When fixed ES or iPS cells are stained using the AP Staining Kit, undifferentiated cells appear red or purple, whereas differentiated cells appear colorless.

 

Product Name: StemAb Alkaline Phosphatase Staining Kit II

Catalog Number: 00-0055

Size

50 assays

Storage and Stability

Store at 4 °C. Do not freeze. Stable for 6 months from date of receipt when stored as directed.

Quality Control

StemAb Alkaline Phosphatase Staining Kit II is functionally tested on human iPS cells and mouse ES cells to ensure product quality.

Kit Contents

  • Fix Solution: 25 mL (Cat. No. 09-0042)
  • AP Staining Solution A: 10 mL (Cat. No. 09-0043)
  • AP Staining Solution B: 10 mL (Cat. No. 09-0065)
  • AP Staining Solution C: 10 mL (Cat. No. 09-0066)

References

  1. Pease, S., Braghetta, P., Gearing, D., Grail, D., and Williams, R.L. (1990) Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev Biol. 141: 344-352.
  2. Smith, A.G., Nichols, J., Robertson, M., and Rathjen, P.D. (1992) Differentiation inhibiting activity (DIA/LIF) and mouse development. Dev. Biol. 151: 339-351.
  3. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
  4. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.

Additional Publications

Top