Stemgent StainAlive TRA-1-81 Antibody (DyLight 488), Mouse anti-Human

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The TRA-1-81 monoclonal antibody reacts with a pluripotent-stem-cell-specific antigen expressed upon the surface of human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific; as human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost, making it a widely used marker to characterize human ES cells and to monitor their differentiation. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide. ES cell morphology, proliferation, and expression of pluripotency markers does not change after staining with this antibody.

 

ICC analysis of TRA-1-81 antibody on primary human iPS cell colonies generated by reprogramming with mRNA. Phase contrast view of cells in culture and the same field of view after staining with StainAlive TRA-1-81 Antibody (DyLight 488) using a 1:100 dilution. [A] Phase contrast view of cells in culture, [B] same field of view after staining with StainAlive TRA-1-81 Antibody (DyLight 488) using a 1:100 dilution.

   

Product Name

StainAlive TRA-1-81 Antibody (DyLight 488), Mouse anti-Human

Catalog Number

09-0069

Size

100 µL

Formulation

Phosphate-buffered solution, pH 7.2, containing no preservative

Storage and Stability

Store at 4 °C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed.

Quality Control

Tested by immunocytochemistry to ensure product quality. Endotoxin level is less than 0.1 EU/µg (0.01 ng/µg) of the protein as determined by the LAL assay.

Recommended Usage

The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. For an application specific protocol, please see  General Protocol for ICC Staining of Live Cells

Concentration

0.5 mg/mL

Isotype

Mouse IgM, κ

Immunogen

Human embryonal carcinoma cell line 2102Ep

Reactivity

Human

Preparation

This antibody was purified by affinity chromatography and conjugated with DyLight 488 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. 0.2 µm filter sterilized. Formulation is free from bacteria, fungi, mycoplasma, and mouse virus

Clone

TRA-1-81

  1. Andrews, P.W., Banting, G., Damjanov, I., Arnaud, D., and Avner, P. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361.
  2. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.
  3. Henderson, J.K., Draper, J.S., Baillie, H.S., Fishel, S., Thomson, J.A., Moore, H., and Andrews, P.W. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337.
  4. Schopperle, W.M., and DeWolf, W.C. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730.

Additional Publications

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