The TRA-1-60 antibody reacts with a pluripotent, stem cell specific antigen expressed on undifferentiated human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers
This antibody is ideal for live-staining cells, because it has been specifically formulated with low endotoxin and no sodium azide. This allows in-process monitoring of reprogramming experiments without sacrificing cells. ES cell morphology, proliferation, and expression of pluripotency markers does not change after staining with StainAlive TRA-1-60 antibody.
Immunocytochemical analysis of TRA-1-60 on H1 human ES cells. Phase contrast view of cells in culture and the same field of view after staining with StainAlive TRA-1-60 Antibody (DyLight 488) using a 1:100 dilution.
StainAlive TRA-1-60 Antibody (DyLight 488), Mouse anti-Human
Phosphate-buffered solution, pH 7.2, containing no preservative
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed.
Tested by immunocytochemistry to ensure product quality. Endotoxin level is less than 0.1 EU/µg (0.01 ng/µg) of the protein as determined by the LAL assay.
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. For an application specific protocol, please see General Protocol for ICC Staining of Live Cells.
Mouse IgM, κ
Human embryonal carcinoma (EC) cell line 2102Ep
This antibody was purified by affinity chromatography and conjugated with DyLight 488 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. 0.2 µm filter sterilized.
Free from bacteria, fungi, mycoplasma, and mouse virus.