Stage-specific embryonic antigen-4 (SSEA-4) is expressed in a variety of human cell lines including embryonic carcinoma (EC), embryonic stem (ES), and induced pluripotent stem (iPS) cells. SSEA-4 is down-regulated upon differentiation, making it a widely used marker to characterize human ES cells and monitor their differentiation. In mouse, SSEA-4 is expressed on oocytes and early cleavage-stage embryos, but not on EC or ES cells. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide. ES cell morphology, proliferation, and expression of pluripotency markers does not change after staining with this antibody.
ICC analysis of SSEA-4 Antibody on H1 human ES cells at 10X magnification. [A] Phase contrast view of hES cell colony in culture, [B] same field of view after staining with StainAlive SSEA-1 Antibody (DyLight 550) using a 1:100 dilution.
StainAlive SSEA-4 Antibody (DyLight 550), Mouse anti-Human
Phosphate-buffered solution, pH 7.2, containing no preservative
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed.
Tested by immunocytochemistry to ensure product quality. Endotoxin level is less than 0.1 EU/µg (0.01 ng/µg) of the protein as determined by the LAL assay.
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. For an application specific protocol, please see General Protocol for ICC Staining of Live Cells
Mouse IgG3, κ
Human embryonal carcinoma cell line 2102Ep
This antibody was purified by affinity chromatography and conjugated with DyLight 550 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. The formulation is 0.2 µm filter sterilized and free from bacteria, fungi, mycoplasma, and virus.