Assay services using Alvetex™
REPROCELL is the leading preclinical and drug discovery CRO offering a wide range of safety, efficacy, and ADME assays in human fresh tissues.
Several of our assays are built upon Alvetex 3D cell culture technology
3D Human Skin Models
Alvetex Scaffold can be used to create 3D, multi-layer full-thickness skin models. Unlike other in vitro skin models, this 3D model possesses a fully developed stratum corneum. This model has been used to study dermal invasion by melanoma cells, giving a good model to study treatments for melanoma. We can run this assay as a service, or you can set it up in your own lab to model skin function and disease. The figure below shows histological analysis of skin equivalents developed using Alvetex compared to our human skin biopsies.
- Ideal for high-throughput projects
- Can be set up in your own laboratory or outsourced
- A budget-friendly alternative to testing in human fresh skin
- Can be used to model early melanoma invasion
Left: Histochemical analysis of our 3D human skin model. Right: Real human skin.
3D Skin Cancer Model
Alvetex Scaffold 3D tissue culture supports have been used for a number of cancer models. Cancer invasion assays measure the effect of potential drugs on the migration of cancer cells into the matrix. Cytotoxicity assays measure the potential anticancer agents to halt or reverse the growth of cancer cells. REPROCELL can perform these assays as a service, as well.
Histochemical analysis revealing migration of melanocytes (indicated by arrow) in our 3D skin model.
3D Autologous Skin Model for toxicity testing
Alcyomics® has developed a full-thickness autologous skin equivalent model from healthy primary human tissue which is representative of normal human skin.
Alvetex Scaffold 3D tissue culture supports have been used for a number of cancer models. Cancer invasion assays measure the effect of potential drugs on the migration of cancer cells into the matrix. Cytotoxicity assays measure the potential anticancer agents to halt or reverse the growth of cancer cells. REPROCELL can perform these assays as a service, as well.
Image: Histochemical analysis revealing migration of melanocytes (indicated by arrow) in our 3D skin model.
Video: How to model cancer cell invasion using novel 3D cell culture technology
3D Human Airway Mucosa Model
REPROCELL’s Alvetex Scaffold inserts (AVP005) can be used to develop a 3D model for human lung mucosa, which shows multiple cell types typically found in the airways. This 3D mucosal model can be extended to create a model for bacterial infection, which allows study of the mechanism and treatment of COPD and otitis media.
Image: Histochemical analysis of our 3D human airway mucosa model.
3D Neurite Outgrowth Model
By measuring neurite outgrowth you can assess the effects of your test articles on neurite formation or toxicity. Our translational neurite outgrowth model combines our award-winning Alvetex scaffold technology with iPSC-derived human neurons to produce a mature, neuronal network that develops dense synaptic connections.
Developed in partnership with the University of Durham, REPROCELL’s iPSC-derived StemRNA™ Neuro cells - available as healthy wild-type and Alzheimer Disease patient-derived (presenilin 2 mutation) - are used to assess pathological changes in vitro. This system is compatible with many physiological and pathological ECM coatings (laminin, CSPGs), known pathway inhibitors, and therapeutic lead compounds.
- Measure inhibition and recovery of neurite outgrowth
- Use iPSC-derived StemRNA™ Neuro cells derived from healthy or Alzheimer Disease donors
- Explore pathological changes in functional human cells in vitro
- Assess physiological and pathological ECM coatings, known pathway inhibitors, and test articles
Image: Immunocytochemistry analysis showing neurites in our 3D outgrowth model.
