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Alvetex Protocols

Alvetex Scaffold

Basics

Alvetex Scaffold Quick Start ProtocolPR-ALVETEX-SCAFFOLD-01-D001
Media Volumes Guide for Different Feeding Options in Alvetex Scaffold in Well Insert formatsPR-ALVETEX-SCAFFOLD-56-D001
Alvetex Scaffold 96-well Plate: Quick Start ProtocolPR-ALVETEX-SCAFFOLD-02-D001
Alvetex Scaffold 96-well plate: Instructions for Use and Example ApplicationsPR-ALVETEX-SCAFFOLD-03-D001
Alvetex Scaffold 384-well Plate: Quick Start ProtocolPR-ALVETEX-SCAFFOLD-04-D001
Alvetex Scaffold 384-well Plate: Instructions for Use and Example ApplicationPR-ALVETEX-SCAFFOLD-05-D001

Coating

Poly-D or L-lysine Coating of Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-06-D001
Collagen I Coating of Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-07-D001
Collagen I Thin Gel Coating of Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-08-D001
Matrigel® Coating of Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-09-D001
Fibronectin Coating of Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-10-D001

Cell-Specific

Guidance notes and protocols for the culture of Primary Rat Hepatocytes in Alvetex Scaffold in well insert and well plate formatsPR-ALVETEX-SCAFFOLD-11-D001
Protocol for the culture of primary rat hepatocytes in Alvetex Scaffold 96-well plate formatPR-ALVETEX-SCAFFOLD-12-D001
Culture of the Canine Kidney Epithelial MDCK Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-13-D001
Example Protocol for the Culture of the MCF-10A Cell Line on Alvetex Scaffold (15mm disc in 12-well insert format, AVP005 and 96-well plate format, AVP009)PR-ALVETEX-SCAFFOLD-14-D001
Example Protocol for the Culture of the CaCo2 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats With or Without a Collagen I Gel LayerPR-ALVETEX-SCAFFOLD-15-D001
Example Protocol for the Co-Culture of the Caco-2 Cell Line with the CCD-18co Cell Line on Alvetex Scaffold in Well Insert FormatsPR-ALVETEX-SCAFFOLD-16-D001
Example Protocol for the Culture of the Breast Carcinoma MDA-MB-231 Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-17-D001
Example Protocol for the Culture of the Human Colon Fibroblastic CCD-18co Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-18-D001
Example Protocol for the Culture of the Lung Carcinoma A549 Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-19-D001
Example Protocol for the Culture of the Breast Invasive Ductal Carcinoma BT-474 Cell Line on Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-20-D001
Example Protocol for the Culture of the PC-3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert FormatsPR-ALVETEX-SCAFFOLD-21-D001
Example Protocol for the Culture of the LN-229 Cell Line on Alvetex Scaffold in 96-well Plate FormatPR-ALVETEX-SCAFFOLD-22-D001
Example protocol for the Culture of the 3T3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert FormatsPR-ALVETEX-SCAFFOLD-23-D001
Example protocol for the culture of the TERA2.cl.SP12 cell line on Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-24-D001
Example protocol for the culture of the HepG2 cell line on Alvetex Scaffold in Well Plate and Well Insert FormatsPR-ALVETEX-SCAFFOLD-25-D001
Example Protocols for the Culture of the CHO-K1 Cell Line on Alvetex Scaffold in Well Plate and Well Insert FormatsPR-ALVETEX-SCAFFOLD-26-D001
Example Preparation of HaCaT Epidermal Equivalent Using Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-27-D001
Development of Full Thickness Human Skin Model Using Alvetex Scaffold TechnologyPR-ALVETEX-SCAFFOLD-28-D001
Example Protocol for the Culture of the SW480 Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-29-D001
Example Protocol for the Culture of the SW620 Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-30-D001
Example Protocols for the Co-Culture of either the SW620 or the SW480 Cell Line with the 3T3 Cell Line on Alvetex Scaffold in Well Insert FormatsPR-ALVETEX-SCAFFOLD-31-D001
Example Protocol for the Culture of the MG63 Cell Line on Alvetex Scaffold in Well Insert FormatsPR-ALVETEX-SCAFFOLD-32-D001
Example Protocol for the Culture of Primary Rat MSCs on Alvetex Scaffold in Well Insert FormatsPR-ALVETEX-SCAFFOLD-33-D001
Example Protocols for the Culture of the MCF-7 Cell Line on Alvetex Scaffold in Well Plate and Well Insert FormatsPR-ALVETEX-SCAFFOLD-34-D001

Transfection

3D Transfection System (Mirus)PR-ALVETEX-SCAFFOLD-35-D001

Cell Visualization

Simple Visualisation of Cells on Alvetex Scaffold Using Light Microscopy (Neutral Red-Methyl Blue Dye)PR-ALVETEX-SCAFFOLD-36-D001
Live Cell Imaging of 3D Cultures Grown on Alvetex Scaffold Using Confocal MicroscopyPR-ALVETEX-SCAFFOLD-37-D001
Wheat Germ Agglutinin Staining of 3D Cultures Grown on Alvetex Scaffold and Alvetex StrataPR-ALVETEX-SCAFFOLD-57-D001

Metabolic Assays

AlamarBlue® Cell Viability Assay of upcyte® hepatocyte Cultures Grown on Alvetex Scaffold in 3DPR-ALVETEX-SCAFFOLD-38-D001
Determination of Cell Number and Growth Characteristics within Alvetex Scaffold using the PicoGreen® AssayPR-ALVETEX-SCAFFOLD-39-D001
XTT Cell Viability Assay for Alvetex Scaffold 96-Well Plate FormatPR-ALVETEX-SCAFFOLD-40-D001
MTS Cell Viability Assay of upcyte® Hepatocyte Cultures Grown on Alvetex Scaffold in 3DPR-ALVETEX-SCAFFOLD-41-D001
MTT cell viability assay of cultures grown on Alvetex Scaffold in 3DPR-ALVETEX-SCAFFOLD-42-D001

Genomics and Proteomics

Total Protein Extraction from Cells Cultured in Alvetex Scaffold in 3DPR-ALVETEX-SCAFFOLD-43-D001
Protein Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate FormatPR-ALVETEX-SCAFFOLD-44-D001
Extraction of RNA from Cells Cultured in Alvetex Scaffold in 3DPR-ALVETEX-SCAFFOLD-45-D001
RNA Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate FormatPR-ALVETEX-SCAFFOLD-46-D001

Histology

Histology Series Part 1: Choosing the Right Fixative to Preserve 3D Cell CulturesPR-ALVETEX-SCAFFOLD-47-D001
Histology Series Part 2: Processing Alvetex Scaffold 3D Cultures for PFA Fixation and Paraffin Wax Embedding for ImmunofluorescencePR-ALVETEX-SCAFFOLD-48-D001
Histology Series Part 3: Processing Alvetex Scaffold 3D Cultures for Bouin’s Fixation and Paraffin Wax Embedding for Haematoxylin and Eosin StainingPR-ALVETEX-SCAFFOLD-49-D001
Histology Series Part 4: Processing Alvetex Scaffold 3D Cultures for Resin Embedding and Toluidine Blue Staining for Light MicroscopyPR-ALVETEX-SCAFFOLD-50-D001
Histology Series Part 5: Processing Alvetex Scaffold 3D Cultures for Scanning Electron Microscopy (SEM)PR-ALVETEX-SCAFFOLD-51-D001

Advanced Microscopy

(See also Histology Series Part 5, above.)
Processing Alvetex Scaffold 3D Cultures for CryosectioningPR-ALVETEX-SCAFFOLD-52-D001
Immunofluorescence Confocal Microscopy of 3D Cultures Grown on Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-53-D001

Cell Retrieval

Retrieval of Cells from Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-54-D001
Protocol for the removal of Alvetex discs from 6-well or 12-well inserts by unclipping the well insert basePR-ALVETEX-SCAFFOLD-55-D001

Alvetex Strata

Alvetex Strata Quick Start ProtocolPR-ALVETEX-STRATA-01-D001
Retrieval of Cells from Alvetex StrataPR-ALVETEX-STRATA-02-D001

 

See also:

Stemgent protocols

REPROCELL protocols and manuals

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