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Alvetex Protocols

Alvetex Scaffold

Basics

Alvetex Scaffold Quick Start ProtocolPR-ALVETEX-SCAFFOLD-D001002-US
Media Volumes Guide for Different Feeding Options in Alvetex Scaffold in Well Insert formatsPR-ALVETEX-SCAFFOLD-D056002-US
Alvetex Scaffold 96-well Plate: Quick Start ProtocolPR-ALVETEX-SCAFFOLD-D002002-US
Alvetex Scaffold 96-well plate: Instructions for Use and Example ApplicationsPR-ALVETEX-SCAFFOLD-D003002-US
Alvetex Scaffold 384-well Plate: Quick Start ProtocolPR-ALVETEX-SCAFFOLD-D004002-US
Alvetex Scaffold 384-well Plate: Instructions for Use and Example ApplicationPR-ALVETEX-SCAFFOLD-D005002-US

Coating

Poly-D or L-lysine Coating of Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-D006002-US
Collagen I Coating of Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-D007002-US
Collagen I Thin Gel Coating of Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-D008002-US
Matrigel® Coating of Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-D009002-US
Fibronectin Coating of Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-D010002-US

Cell-Specific

Guidance notes and protocols for the culture of Primary Rat Hepatocytes in Alvetex Scaffold in well insert and well plate formatsPR-ALVETEX-SCAFFOLD-D011002-US
Protocol for the culture of primary rat hepatocytes in Alvetex Scaffold 96-well plate formatPR-ALVETEX-SCAFFOLD-D012002-US
Culture of the Canine Kidney Epithelial MDCK Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-D013002-US
Example Protocol for the Culture of the MCF-10A Cell Line on Alvetex Scaffold (15mm disc in 12-well insert format, AVP005 and 96-well plate format, AVP009)PR-ALVETEX-SCAFFOLD-D014002-US
Example Protocol for the Culture of the CaCo2 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats With or Without a Collagen I Gel LayerPR-ALVETEX-SCAFFOLD-D015002-US
Example Protocol for the Co-Culture of the Caco-2 Cell Line with the CCD-18co Cell Line on Alvetex Scaffold in Well Insert FormatsPR-ALVETEX-SCAFFOLD-D016002-US
Example Protocol for the Culture of the Breast Carcinoma MDA-MB-231 Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-D017002-US
Example Protocol for the Culture of the Human Colon Fibroblastic CCD-18co Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-D018002-US
Example Protocol for the Culture of the Lung Carcinoma A549 Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-D019002-US
Example Protocol for the Culture of the Breast Invasive Ductal Carcinoma BT-474 Cell Line on Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-D020002-US
Example Protocol for the Culture of the PC-3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert FormatsPR-ALVETEX-SCAFFOLD-D021002-US
Example Protocol for the Culture of the LN-229 Cell Line on Alvetex Scaffold in 96-well Plate FormatPR-ALVETEX-SCAFFOLD-D022002-US
Example protocol for the Culture of the 3T3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert FormatsPR-ALVETEX-SCAFFOLD-D023002-US
Example protocol for the culture of the TERA2.cl.SP12 cell line on Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-D024002-US
Example protocol for the culture of the HepG2 cell line on Alvetex Scaffold in Well Plate and Well Insert FormatsPR-ALVETEX-SCAFFOLD-D025002-US
Example Protocols for the Culture of the CHO-K1 Cell Line on Alvetex Scaffold in Well Plate and Well Insert FormatsPR-ALVETEX-SCAFFOLD-D026002-US
Example Preparation of HaCaT Epidermal Equivalent Using Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-D027002-US
Development of Full Thickness Human Skin Model Using Alvetex Scaffold TechnologyPR-ALVETEX-SCAFFOLD-D028002-US
Example Protocol for the Culture of the SW480 Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-D029002-US
Example Protocol for the Culture of the SW620 Cell Line on Alvetex Scaffold in Well Insert and Well Plate FormatsPR-ALVETEX-SCAFFOLD-D030002-US
Example Protocols for the Co-Culture of either the SW620 or the SW480 Cell Line with the 3T3 Cell Line on Alvetex Scaffold in Well Insert FormatsPR-ALVETEX-SCAFFOLD-D031002-US
Example Protocol for the Culture of the MG63 Cell Line on Alvetex Scaffold in Well Insert FormatsPR-ALVETEX-SCAFFOLD-D032002-US
Example Protocol for the Culture of Primary Rat MSCs on Alvetex Scaffold in Well Insert FormatsPR-ALVETEX-SCAFFOLD-D033002-US
Example Protocols for the Culture of the MCF-7 Cell Line on Alvetex Scaffold in Well Plate and Well Insert FormatsPR-ALVETEX-SCAFFOLD-D034002-US

Transfection

3D Transfection System (Mirus)PR-ALVETEX-SCAFFOLD-D035001-MIRUS

Cell Visualization

Simple Visualisation of Cells on Alvetex Scaffold Using Light Microscopy (Neutral Red-Methyl Blue Dye)PR-ALVETEX-SCAFFOLD-D036002-US
Live Cell Imaging of 3D Cultures Grown on Alvetex Scaffold Using Confocal MicroscopyPR-ALVETEX-SCAFFOLD-D037002-US
Wheat Germ Agglutinin Staining of 3D Cultures Grown on Alvetex Scaffold and Alvetex StrataPR-ALVETEX-SCAFFOLD-D057002-US

Metabolic Assays

AlamarBlue® Cell Viability Assay of upcyte® hepatocyte Cultures Grown on Alvetex Scaffold in 3DPR-ALVETEX-SCAFFOLD-D038002-US
Determination of Cell Number and Growth Characteristics within Alvetex Scaffold using the PicoGreen® AssayPR-ALVETEX-SCAFFOLD-D039002-US
XTT Cell Viability Assay for Alvetex Scaffold 96-Well Plate FormatPR-ALVETEX-SCAFFOLD-D040002-US
MTS Cell Viability Assay of upcyte® Hepatocyte Cultures Grown on Alvetex Scaffold in 3DPR-ALVETEX-SCAFFOLD-D041002-US
MTT cell viability assay of cultures grown on Alvetex Scaffold in 3DPR-ALVETEX-SCAFFOLD-D042002-US

Genomics and Proteomics

Total Protein Extraction from Cells Cultured in Alvetex Scaffold in 3DPR-ALVETEX-SCAFFOLD-D043002-US
Protein Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate FormatPR-ALVETEX-SCAFFOLD-D044002-US
Extraction of RNA from Cells Cultured in Alvetex Scaffold in 3DPR-ALVETEX-SCAFFOLD-D045002-US
RNA Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate FormatPR-ALVETEX-SCAFFOLD-D046002-US

Histology

Histology Series Part 1: Choosing the Right Fixative to Preserve 3D Cell CulturesPR-ALVETEX-SCAFFOLD-D047002-US
Histology Series Part 2: Processing Alvetex Scaffold 3D Cultures for PFA Fixation and Paraffin Wax Embedding for ImmunofluorescencePR-ALVETEX-SCAFFOLD-D048002-US
Histology Series Part 3: Processing Alvetex Scaffold 3D Cultures for Bouin’s Fixation and Paraffin Wax Embedding for Haematoxylin and Eosin StainingPR-ALVETEX-SCAFFOLD-D049002-US
Histology Series Part 4: Processing Alvetex Scaffold 3D Cultures for Resin Embedding and Toluidine Blue Staining for Light MicroscopyPR-ALVETEX-SCAFFOLD-D050002-US
Histology Series Part 5: Processing Alvetex Scaffold 3D Cultures for Scanning Electron Microscopy (SEM)PR-ALVETEX-SCAFFOLD-D051002-US

Advanced Microscopy

(See also Histology Series Part 5, above.)
Processing Alvetex Scaffold 3D Cultures for CryosectioningPR-ALVETEX-SCAFFOLD-D052002-US
Immunofluorescence Confocal Microscopy of 3D Cultures Grown on Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-D053002-US

Cell Retrieval

Retrieval of Cells from Alvetex ScaffoldPR-ALVETEX-SCAFFOLD-D054002-US
Protocol for the removal of Alvetex discs from 6-well or 12-well inserts by unclipping the well insert basePR-ALVETEX-SCAFFOLD-D055002-US

Alvetex Strata

Alvetex Strata Quick Start ProtocolPR-ALVETEX-STRATA-D001002-US
Retrieval of Cells from Alvetex StrataPR-ALVETEX-STRATA-D002002-US

 

See also:

Stemgent protocols

REPROCELL protocols and manuals

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