StemFit Basic 02 Cell Culture Medium

Catalog ASB01
Size 500 mL
Price Price on Request
$300.00
Availability In stock
Catalog
ASB01

Product Overview

StemFIt Basic 02 is a cell culture medium optimized for iPS cell expansion and maintenance. This medium supports cultivation of cells in both attached and suspension format. The medium also enables single cell passaging of iPSC (recommended to supplement medium with Y27632 Rock Inhibitor; Cat. No. 04-0012). For complete medium preparation, two separate components need to be mixed, and the addition of bFGF (03-0002) is also required (not supplied with this product).



StemFit ASB01 medium is a product configured for sale in the USA and European market. See StemFit AK02N for a configuration sold in Japan and Asia. This product is manufactured by Ajinomoto Company (Japan).

Product Specifications

Product Name
StemFit Basic 02 Cell Culture Medium
Catalog Number
ASB01
Size
500 mL
Storage and Stability
Store components separately as indicated (-20 °C); store reconstituted medium at 4℃
Sterility
sterile
Related Products
StemFit AK02N medium is a functionally equivalent formulation sold in Japan.
Recommended Usage
For cultivation of stem cells or iPSCs
System Components
The product consists of 2 components; a base medium (400 mL), and an additive (100 mL)
Manufacturer
Ajinomoto Co. (Japan)


  1. Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S. “A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.” Sci Rep. 2014 Jan 8;4:3594. doi: 10.1038/srep03594. PubMed PMID: 24399248; PubMed Central PMCID: PMC3884228.
  2. Daisuke Doi, et al.Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation Stem Cell Reports. 2014 Mar 11; 2(3): 337-350.
  3. Takuji Kawamura, et al. Cardiomyocytes Derived from MHC-Homozygous Induced Pluripotent Stem Cells Exhibit Reduced Allogeneic Immunogenicity in MHC-Matched Non-human Primates Stem Cell Reports. 2016 Mar 8; 6(3): 312-320.
  4. Akihiro Arakawa, et al. Speciation of Intracellular Zn, Fe and Cu within both iPS Cells and Differentiated Cells Using HPLC Coupled to ICP-MS J Anal Bioanal Tech. 2016 7:295.
  5. Taizo Ishikawa, et al. Genetic and pharmacological correction of aberrant dopamine synthesis using patient iPSCs with BH4 metabolism disorders. Human Molecular Genetics 25:5188 (2016).
  6. Naoki Ichiyanagi, et al. Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells Stem Cell Reports. 2016 Apr 12; 6(4): 496-510.
  7. Shin-Il Kim, et al. Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons Methods Mol Biol. 2016;1357:111-31.

                Product Specifications

                Product Name
                StemFit Basic 02 Cell Culture Medium
                Catalog Number
                ASB01
                Size
                500 mL
                Storage and Stability
                Store components separately as indicated (-20 °C); store reconstituted medium at 4℃
                Sterility
                sterile
                Related Products
                StemFit AK02N medium is a functionally equivalent formulation sold in Japan.
                Recommended Usage
                For cultivation of stem cells or iPSCs
                System Components
                The product consists of 2 components; a base medium (400 mL), and an additive (100 mL)
                Manufacturer
                Ajinomoto Co. (Japan)

                1. Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S. “A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.” Sci Rep. 2014 Jan 8;4:3594. doi: 10.1038/srep03594. PubMed PMID: 24399248; PubMed Central PMCID: PMC3884228.
                2. Daisuke Doi, et al.Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation Stem Cell Reports. 2014 Mar 11; 2(3): 337-350.
                3. Takuji Kawamura, et al. Cardiomyocytes Derived from MHC-Homozygous Induced Pluripotent Stem Cells Exhibit Reduced Allogeneic Immunogenicity in MHC-Matched Non-human Primates Stem Cell Reports. 2016 Mar 8; 6(3): 312-320.
                4. Akihiro Arakawa, et al. Speciation of Intracellular Zn, Fe and Cu within both iPS Cells and Differentiated Cells Using HPLC Coupled to ICP-MS J Anal Bioanal Tech. 2016 7:295.
                5. Taizo Ishikawa, et al. Genetic and pharmacological correction of aberrant dopamine synthesis using patient iPSCs with BH4 metabolism disorders. Human Molecular Genetics 25:5188 (2016).
                6. Naoki Ichiyanagi, et al. Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells Stem Cell Reports. 2016 Apr 12; 6(4): 496-510.
                7. Shin-Il Kim, et al. Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons Methods Mol Biol. 2016;1357:111-31.