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Flow cytometry consignment services

Services for researchers

Sorting

Introduction

Sorting is a technique that allows collecting the targeted cells only.
Based on this technique, it is possible to develop a new research phase using the targeted cells only.

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Development of sorting

Principle

Principle

It is possible to isolate and collect only targeted cells based on the information obtained through FCM analysis.
A liquid drop is formed by applying oscillation on a jet flow nozzle with a piezoelectric element.

By charging the solution immediately before the formation of a liquid drop that includes the targeted cells, the liquid drop is charged.
Because the orbit of the drop falling from the nozzle is bent by a deflection plate, only the charged drops can be collected separately.

Applications

Cell purification:

  • Purification of hematopoietic stem cell
  • Fractional collection of each blood cell to minimize contamination for various types of experiments.
  • Targeted collection of biocells expressing GPCR or cell membrane protein.
    • Establishment of GPCR express cell line. (Dimer formation GPCR is also available.)
    • Fractional collection of cells for chemical evaluation from primary culture cells after implementation of HTS by pharmaceutical companies. (Our products are delivered in culture flasks or multi-well plates.)
    • Selection of organ-originated cells to establish ES cells, Functional cells derived from Primate ES cells.
    • Gene expression by fractional collection of biocells using cell surface markers, and preparation of cells used for analysis of protein expression.
  • Application for purification and cultivation of yeast, actinomycetes and fungi in producing nature-derived products and in fermentation studies. We promise exceptional speed in our purification treatment compared to conventional treatments.

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The scope of our consignment services
  • Sorting of bone marrow stem cells
  • Sorting of GFP negative cells
  • Sorting of leukemia cells
  • Cell line preparation

Sorting of neutrophil

Sorting of neutrophil

Sorting of hematopoietic stem cells

Sorting of hematopoietic stem cells

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