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Research reagents
Culture Kit for Human and Monkey ES cells
Our "Culture kit for human and monkey ES cells" was developed by a collaboration of ReproCELL Inc. and Nakatsuji laboratory in Kyoto University.
Several products for mouse ES cells are already commercially available. However, when these products are used for human and monkey ES cells, many problems occur such as the differentiation and cell death. Our "Culture kit for human and monkey ES cells" is specialized for human and monkey ES cells.
Medium for human and monkey ES cells
Volume: 500 mL and 250 mL, Storage: -20 degC
This culture medium can be used for not only primate ES cells but also Human iPS cells.　Cell 131, 1-12 (2007)
Advantages
- Long experience in culturing human and monkey ES cells without any abnormality (at least for more than 2 years).
- Lot-to-lot inspection by using monkey ES cell.
- Lot-to-lot inspection of osmolality, pH, sterilization and mycoplasma.
- Ready-to-use.
- Serum free.
Colony of Cynomolgus ES cells
When monkey ES cells are cultured in medium commercially available for mouse ES cells, they start to be differentiated with the colony collapsed.
Dissociation solution for human and monkey ES cells
Volume: 30 mL, Storage: -20 degC
Advantages
- Easy and efficient operation (5min) for passage of large quantities of human/monkey ES cells.
- No mechanical operation with tweezers or knife is required for the ES cell detachment (just pipetting).
- Extremely high viability of human and monkey ES cells (10 times as high as Trypsin).
- Long experience in culturing human and monkey ES cells without any abnormality (at least for more than 2 years).
- Lot-to-lot inspection by using monkey ES cell.
- Lot-to-lot inspection of osmolality, pH, sterilization and mycoplasma.
- Ready-to-use.
- Serum free.
Our dissociation solution shows 10 times as high viability of ES cells as 0.25% Trypsin-EDTA.
Freezing medium for human and monkey ES cells
Volume: 25 mL, Storage: -80 degC
Advantages
- Extremely high viability of human and monkey ES cells (more than 10%), compared to DMSO (below 0.5%).
- Long time cryopreservation in below -135 deg C freezer.
- Lot-to-lot inspection by using monkey ES cell.
- Lot-to-lot inspection of osmolality, pH, sterilization and mycoplasma.
- Ready-to-use.
- Serum free.
Feeder Cells (SL10)
Advantages
- Available for mouse, monkey, and human ES cells as feeder cell.
- Neomycin resistance.
- Less lot-to-lot variation because it is cell line.
- Mitomycin C treatment.
- Ready-to-use.
