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Drug Discovery
High-throughput screening (HTS) has become a powerful procedure in the pharmaceutical industry for a variety of applications. Instrumental improvements in recent years have contributed significantly to this development. However, we realize biological improvements still remaining.
To reach beyond HTS, ReproCELL provides several categories of cells.
A lot of hit-compounds including false positive, indicating poor-specificity and faint activity are still in your lab after HTS. Our cells can be applied to HTS, Hit-to-Lead, and Hit-optimization to obtain true hit compounds quickly and more reliable.
- Primary Culture Cells
- ES cells, Functional cells derived from Primate ES cells
- Reprogrammed cells
Primary culture cells
Primary culture cells can be used for cell based assay in drug discovery. Although it is relatively hard to use primary cells compare to usual cell lines, primary cells are powerful tool for post-HTS or later stage.
ReproCELL offers primary cultured cells which are very difficult to prepare for drug discovery.
Rat lower intestinal GLP-1 secreting cells
Glucagon-like peptide-1 (GLP-1) is derived from the transcription product of the proglucagon gene. The major source of GLP-1 in the body is the intestinal L cell that secretes GLP-1 as a gut hormone. The biologically active forms of GLP-1 are: GLP-1-(7-37) and GLP-1-(7-36)NH2.
GLP-1 secretion by L cells is dependant on the presence of nutrients in the lumen of the small intestine. Lipids are the major secretagogue (an agent that causes or stimulates secretion) of this hormone.
GLP-1 functions to decrease feed intake, to increase the expression of the insulin gene, and to increase secretion of insulin. As an incretin, GLP-1 increases insulin secretion from the pancreas in a glucose-dependent manner. In addition to this, GLP-1 also possesses several other physiological properties that make it a candidate for the potential treatment of diabetes mellitus.
ReproCELL offers primary culture rat intestinal cells secreting GLP-1. GLP-1 level in media after stimulation will reach the range of usual GLP-1 kit.
Rat lower intestinal GLP-1 secreting cells (crude)
1: 5.6 mM Glucose (control)
2: 25 mM Glucose
3: 10 uM Lithocholic acid acid (w/ 5.6 mM Glucose )
4: 100 uM Lithocholic acid acid (w/ 5.6 mM Glucose )
5: 10 uM Linoleic acid (w/ 5.6 mM Glucose )
6: 100 uM Linoleic acid (w/ 5.6 mM Glucose
Rat intestinal GIP secreting cells
Glucose-dependent insulinotropic polypeptide(GIP)
GIP is a 42-amino acid gastrointestinal regulatory peptide that stimulates insulin secretion from pancreatic beta cells in the presence of glucose.
GIP is a gastrointestinal hormone that is secreted in response to food intake and modulates beta cell function. It may also regulate beta-cell fate. Released from the nutrient-sensing K-cells of the upper intestine, GIP acts on various tissues, including pancreatic beta cells, via interaction with its G-protein-coupled receptor.
ReproCELL offers primary culture rat intestinal cells secreting GIP. GIP level in media after stimulation will reach the range of usual GLP-1 kit.
Rat intestinal GIP secreting cells
Rat intestinal GIP secreting cell kit
1: 5.6 mM Glucose
2: 22.4 mM Glucose
3: 10 uM Linoleic acid (w/ 5.6 mM Glucose )
4: 100 uM Linoleic acid (w/ 5.6 mM Glucose )
5: 10 nM Bombesin (w/ 5.6 mM Glucose )
6: 100 nM Bombesin (w/ 5.6 mM Glucose )
Normalization using the level of GIP in 100 nM Bombesin (w/ 5.6 mM Glucose )