ReproNeuro DA kit: Dopaminergic Neural Progenitors

Source of human iPSC-derived neurons for basic research and drug discovery

  • Reliable source of human dopaminergic neurons
  • Disease model generation by DNA recombination
  • For efficient neurotoxicity assays and drug discovery
  • Expression of dopaminergic neuron markers are confirmed
  • Dopamine release and reuptake are observed
  • All lots are quality checked by functional assays

ReproNeuroDA kit

The cells provided are neural progenitors. After two weeks culture using the attached Maturation Medium dopaminergic neurons will be obtained. The dopaminergic neurons can be used effectively for study of the CNS disease such as Parkinson’s disease.

Instruction manual for RCESD010 ReproNeuro DA kit (Jump to the download link, open in a new window)
Instruction manual for RCESD011 ReproNeuro DA kit small pack (Jump to the download link, open in a new window)

Instruction Movie for thawing and seeding ReproNeuro DA kit (Jump to the Movie, open in a new window)

 

Expression of dopaminergic neuron markers

Dopaminergic neurons expressing both bIII-tubulin (a neuron marker, shown in green) and TH (a dopaminergic neuron marker, shown in red) are obtained after a 14-day culture.
Dopamine release by dopaminergic neurons derived from human iPS cells

The dopamine release from the differentiated neuron derived from human iPS cells was demonstrated, since dopamine concentration in the culture medium was increased by depolarization (elevate the KCl concentration to 56 mM).

 Dopamine release in the medium
Dopamine uptake by dopaminergic neurons derived from human iPS cells
The differentiated neuron derived from human iPS cells were treated with DMSO or DAT (Dopamine Transporter) inhibitor (GBR12909, 20 µM). Dopamine uptake was measured by Neurotransmitter transporter uptake assay kit (Molecular Device). The fluorescence intensity in control was reduce by DAT inhibitor treatment. That results indicate functional expression of the neurotransmitter transporter.
+DMSO +GBR12909 Fluorrescence intensity
Customized transgenic human neurons for disease modeling

ReproCELL has developed iPS cell lines which enable quick and reliable production of transgenic neurons. Using this technology, human disease model neurons with background isogenic to control neurons can be easily obtained, which was not possible in the past. To learn more, visit our technical resource web page.

Transgenic Neurons
Product list
Catalog No. Product Price
RCESD010 ReproNeuro DA: 3 x 106 cells ASK
RCESD011 ReproNeuro DA small pack: 3 x 105 cells ASK
Transgenic model neuron generation service ASK
For replenishing the reagents included in the kits
RCESD013 DA Maturation kit
(Culture medium kit for extended culture of RCESD010)

  • Maturation Medium (40mL)
  • Additive A (520μL)
 ASK
RCESD014 DA Maturation kit small
(Culture medium kit for extended culture of RCESD011)

  • Maturation Medium (5mL)
  • Additive A (100μL)
 ASK
RCESD015 DA Thawing Medium (30mL)
(Thawing Medium for RCESD010/011)		 ASK
RCESD016 DA Coat (150μL)
(Coating Solution for RCESD010/011)		 ASK

 

Kit contents & storage condition
RCESD010 ReproNeuro DA kit: contents

  • human iPS cell-derived neural progenitors
    3 x 106 cells (1 x 106 cells/vial x 3 vials)
    (frozen cells, for plating one whole 96-well plate)
  • Thawing Medium (30 mL)
  • Maturation Medium (40 mL)
  • Additive A (520 µL)
  • Coating Solution (150 µL)
 RCESD011 ReproNeuro DA kit small pack: contents

  • human iPS cell-derived neural progenitors
    3 x 105 cells (3 x 105 cells/vial x 1 vial)
    (frozen cells, for plating ~ 10 wells of a 96-well plate)
  • Thawing Medium (10 mL)
  • Maturation Medium (5 mL)
  • Additive A (100 µL)
  • Coating Solution (15 µL)

Human dompaminergic neurons are obtained by differentiating from the provided neural progenitor cells using maturation medium and additive A. The human dopaminergic neurons can be used effectively for research and drug discovery of CNS diseases including Parkinson’s disease.

ReproNeuro DA kit

Storage condition Cells: -80 degC
Other reagents: -20 degC
Shipping with dry ice

 

Data Sheets

< RCESD010: ReproNeuro DA kit >

 
< RCESD011: ReproNeuro DA kit small pack >

Page Top