• QT prolongation assay service using human cardiomyocytes derived from iPS cells
• ECG-like recordings by using extracellular electrodes
• Results in excellent agreement with clinical findings
• Ideal as Post HTS/Hit-to-Lead non-GLP test
• Covers all cardiac ion channels including hERG channel
• Screens non-ion channel factors as well
• Also for beat rate change detection

QTempo is the pre-clinical functional assay that can capture the whole complexity of cardiac activity in vitro. By making ECG-like recordings composed of QRS complex and T wave, QTempo can capture the critical cardiac parameters including QT interval and beat rate.
  The cardiomyocyte clusters used in QTempo generate robust, consistent data over extended periods. This allows a wide variety of drug concentrations to be sequentially tested, increasing confidence in drug safety before further development. QTempo has generated results in complete agreement with clinical findings, even with the cardiotoxic compounds not identified by hERG assay.

The actual data obtained by QTempo assay is shown here.

The cardiomyocyte clusters used in our assay service are strictly quality-checked before use.

• Size of the cell clump: 100-300 um （about 10,000 cells)
• Amplitude of Na+ amplitude: 40 uV or larger
• Amplitude of K+ amplitude: 2 uV or larger
• Interval between K+-wave and Na+-wave: < 600 msec
• Beat rate: 30~60 beats/minute
  (average of 3 minute measurement of untreated cells)
• Variation of beat rate: < 10 bpm