ReproCardio 2: Frozen Human Cardiomyocytes (New Product)

Frozen human iPSC-derived cardiomyocytes for 96 well high throughput & high content assays

  • Frozen single cells with structural/functional integrity
  • Can be used as monolayers and clumps according to your purpose
    in a 96 well plate format
  • For efficient in-vitro cardiotoxicity assays and drug discovery
  • Functionally charecterized using various reference compounds
  • Expresses cardiac ion channels and cardiomyocyte-specific markers
  • The assay medium for extracellular recordings and live imaging is available separately

ReproCardio 2
* ReproCardio 2 is a frozen product. If you are interested in our fresh beating cardiomyocyte clusters, please check another product ReproCardio. Otherwise, if you are interested in our assay service using ReproCardio please refer to QTempo web page.

RCESD008 ReproCardio2 instruction Manual (Jump to the download link, open in a new window)
RCESD009 ReproCardio2 small pack instruction Manual (Jump to the download link, open in a new window)

Instruction Movie for thawing and seeding ReproCardio2 (Jump to the Movie, open in a new window)

 

Convenient frozen cells for wide range of applications

ReproCardio 2 cardiomyocytes are delivered as frozen single cells. Using the specially-developed plates included in the kit, you can re-constitute the cells into cell monolayers or clumps depending on your purpose of research. The re-constituted monolayers and clumps beat periodically and robustly which provide excellent in vitro models of in vivo heart. Visit here for the details.

   
ECG-like recordings using clumps
Live imaging using monolayers
Experiments using single cells

 

Characterization and application of ReproCardio 2 cardiomyocytes

ReproCardio 2 cardiomyocytes are delivered frozen, but even after thawing they retain structural integrity and function thanks to our specially developed freezing technology. Please refer to the link below for the detailed characterization results. Visit here for the details.

Marker gene expression   Beating and electrical activity   Accurate replication of in vivo drug effects
 

 

 

Product list
Catalog No. Product Price
RCESD008 ReproCardio 2: 1 x 106 cells ASK
RCESD009 ReproCardio 2 small pack: 1 x 105 cells ASK
For replenishing the culture medium or for functional assays, please use the media listed below.
RCESD012 ReproCardio culture medium 2 (80mL) ASK
RCESD003 ReproCardio assay medium (100 mL) ASK

 

Kit contents & storage condition
RCESD008 ReproCardio 2 contents

  • human iPS cell-derived single cardiomyocytes
    1 x 106 cells (3.4 x 105 cells/vial x 3 vials)
    (frozen cells, for making 100 clumps or thin layers)
  • ReproCardio Culture Medium 2 (FBS-)
    80 mL x 2 bottles
    (Need to be supplemented with 20mL FBS before use)
  • Coating Solution
    30 mL x 1 bottle
    (for preparing thin layers and culture single cells)
  • 96 well plate for preparing cell clumps x 1
  • 96 well plate for preparing cell monolayers x 1
 RCESD009 ReproCardio 2 small pack contents

  • human iPS cell-derived single cardiomyocytes
    1 x 105 cells (1 x 105 cells/vial x 1 vial)
    (frozen cells, for making 10 clumps or thin layers)
  • ReproCardio Culture Medium 2 (FBS-)
    80 mL x 1 bottle
    (Need to be supplemented with 20mL FBS before use)
  • Coating Solution
    30 mL x 1 bottle
    (for preparing thin layers and culture single cells)
  • 96 well plate for preparing cell clumps x 1
  • 96 well plate for preparing cell monolayers x 1

For extracellular electrophysiological recordings using a multi electrode array system or live fluorescence imaging, our assay medium is available separately.

Storage condition Cells: -80 degC, or liq. N2 for long time storage
ReproCardio Culture Medium 2: -20 degC
After thawing the medium, keep it at 4 degC and use it up within 2 weeks.
Coating solution: Room temperature or 4 degC

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