ReproStem

Culture Medium for reliable, simple and economical maintenance of human ES/iPS cells

  • Economical medium for human ES/iPSC feeder-dependent culture
  • Reasonable price for everyday use
  • Developed based on Primate ES Cell Medium
  • Lot-to-lot control of other critical criteria including osmolarity, pH, sterility and mycoplasma
  • Serum-free
ReproStem

ReproStem is a high quality, serum-free economical formulation based on the popular Primate ES Cell Medium jointly developed by ReproCELL and Kyoto University. Primate ES Cell Medium enabled development of the world’s first human iPS cells in 2007 by Prof Shinya Yamanaka and his colleagues. Following this breakthrough, ReproCELL’s Primate ES Medium has been successfully adopted for culture of ES and iPS cells by leading labs throughout the world. ReproStem has been specifically developed with a reformulation of the same quality components to reduce the cost. Now you can enjoy reliable ES and iPS cell development for human and other primate cells whilst maintaining the convenience. ReproStem simply requires the addition of bFGF prior to use*.

*: not necessary for all the cell lines

The instruction manual of ReproStem (PDF, open in a new window)
Instruction Movie for human iPS cell culture (Jump to the Movie, open in a new window)

 

Maintenance of an undifferentiated state using ReproStem

ReproStem is sutable for keeping human ES/iPS cells in an undifferentiated state.

Human ES cell line KhES-4 cultured using ReproStem with MEF was examined for the morphology (left) and alkaline phosphatase activity (right). The growth was slightly slower than in Primate ES Cell Medium but the cells have grown without any abnormalities. Examined by Kyoto University.

 

Product list
Catalog No. Product Retail Price Quantity Purchase
RCHEMD005 ReproStem (500mL) x 1 $150.00

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RCHEMD005A ReproStem (500mL) x 5 $675.00

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RCHEMD005B ReproStem (500mL) x 5 & bFGF (25ug) pack $725.00

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Payment method can be chosen from Paypal / credit card / bank transfer.

 

Data sheets

The data sheet for each lot of the product (open in a new window)