Differentiation of human iPS cells into cardiomyocytes

Human iPS cells cultured with ReproFF2 (upper row) could differentiate into cardiomyocytes, similarly to those cultured with the feeder cells (lower row). The cell clumps were beating, and the morphology and the expression of the cardiomyocyte markers were confirmed as shown.

※α-MHC, Connexin-43, Cardiac Troponin T: heart muscle cell markers

Differentiation of human iPS cells into neurons

Human iPS cells cultured with ReproFF2 (upper row) could differentiate via rosettes into dopaminergic neurons, as those cultured with feeder cells (lower row). The expression of betaIII-tubulin was observed in neurites, and expression of tyrosine hydroxylase (TH, the enzyme for dopamine biosynthesis) was observed. Dopamine release and uptake were also confirmed.

※rossttes: morphological characteristics of neural precursor
※βIII-tubulin: a neurite marker
※TH(Tyrosine 3-Hydroxyrase): a dopaminergic neuron marker