A New Feeder-Free Medium for human ES/iPS cells

Culture maintenance required only three days/week Halves the amount of media use Reduce the labor cost for the weekend Maintains stable undifferentiated state Maintains robust differentiation capability No need of mechanical manipulation for passaging when used with Laminin-5
ReproFF2 is a new, improved feeder-free medium for human ES/iPS cells. Unlike conventional culture media that requires changing on a daily basis, ReproFF2 needs culture maintenance only three days a week, with no compromise made to the quality of the cells. Change the medium on Monday and Wednesday, passage the cells on Friday. Consequently, the cost and amount of medium used are halved, feeder cells need not be prepared, and the culture need not be cared during the weekend.   ReproFF2 has been developed to reduce the number of steps in the workflow and is in a highly convenient ready-to-go formulation. Just warm up, add bFGF*, and ReproFF2 is ready.
	human iPS cells (201B7) grown with ReproFF2 on a Laminin-5 coated plate.
*: not necessary for all the cell lines

The instruction manual for ReproFF2 (PDF, open in a new window)
Instruction Movie for human iPS cell feeder-free culture (Jump to the Movie, open in a new window)

Improved usability

With ReproFF2, medium change is not required everyday.
Culture doesn’t have to be cared during the weekend if passaging is done on Friday.

FAQ

The answers to frequently asked questions are here.

Robust maintenance of an undifferentiated state and an ability to differentiate

ReproFF2 is suitable for keeping human ES/iPS cells stably in an undifferentiated state.
The undifferentiated cells also retain the ability to differentiate into specific cell types.

Maintenance of an undifferentiated state of human iPSC
	ReproFF2 is suitable for maintenance of an undifferentiated state of human iPS cells without feeders. Check the detailed results.
Maintenance of an undifferentiated state of human ESC
	ReproFF2 is suitable also for maintenance of an undifferentiated state of human ES cells without feeders. Check the detailed results. In collaboration with Kyoto University.
Retention of differentiation capability
	Human iPS cells cultured using ReproFF2 could differentiate into cardiomyocytes and neurons. Check the detailed results.

Stability of the cells cultured in ReproFF2

ReproFF2 can maintain the undifferentiated human ES/iPS cells stably in the long term.
Please watch the movies of the growing cells.

Product list

Data Sheets

• Lot: R6M110309A
• Lot: R6M110629