ReproFF

Feeder-Free Medium for human ES/iPS Cells

  • Easy adaptation from feeder-dependent culture
  • Enables superior expansion of human ES/iPS cells
  • Maintains normal morphology and differentiation capability
  • Each lot is performance qualified using human iPS cells
  • No need of mechanical manipulation for passaging when used with Laminin-5
ReproFF

ReproFF is a serum- and feeder-free medium developed in collaboration with leading stem cell scientists to allow trouble-free, reproducible maintenance of ES and iPS cells without the requirement for a feeder layer. Manufactured to the same high standards as our other products, ReproFF has been formulated for the growth and expansion of human ES/iPS cells. ReproFF has been developed to reduce the number of steps in your workflow and is in a highly convenient ready-to-go formulation. Just warm up, add bFGF*, and ReproFF is ready.

*: not necessary for all the cell lines

ReproFF

Alkaline phosphatase activity examined in human iPS cells cultured using ReproFF with 5 ng/mL bFGF for 5 passages

The instruction manual of ReproFF (PDF, open in a new window)
Instruction Movie for human iPS cell feeder-free culture (Jump to the Movie, open in a new window)

 

Maintenance of an undifferentiated state without feeders using ReproFF

ReproFF is sutable for keeping human ES/iPS cells in an undifferentiated state without the need of the feeder cells.

Human ES cell line KhES-1 cultured using ReproFF without feeder cells was examined for the morphology (left) and the expression of Oct-3/4, a pluripotency marker gene (right). The cells have grown without any abnormalities. Examined by Kyoto University.

 

[Movie] human iPSC growing in ReproFF

 

Product list
Catalog No. Product Retail Price Quantity Purchase
RCHEMD004 ReproFF (500mL) x 1 $230.00

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RCHEMD004A ReproFF (500mL) x 5 $1035.00

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RCHEMD004B ReproFF (500mL) x 5 & bFGF (25ug) pack $1085.00

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RCHEMD004C ReproFF (500mL) & Laminin-5 (1ug) x 30 pack $830.00

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Payment method can be chosen from Paypal / credit card / bank transfer.

 

Data sheet

The data sheet for each lot of the product (open in a new window)