ReproFF
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ReproFF is a serum- and feeder-free medium developed in collaboration with leading stem cell scientists to allow trouble-free, reproducible maintenance of ES and iPS cells without the requirement for a feeder layer. Manufactured to the same high standards as our other products, ReproFF has been formulated for the growth and expansion of human ES/iPS cells. ReproFF has been developed to reduce the number of steps in your workflow and is in a highly convenient ready-to-go formulation. Just warm up, add bFGF*, and ReproFF is ready.
*: not necessary for all the cell lines |
Alkaline phosphatase activity examined in human iPS cells cultured using ReproFF with 5 ng/mL bFGF for 5 passages |
The instruction manual of ReproFF (PDF, open in a new window)
Instruction Movie for human iPS cell feeder-free culture (Jump to the Movie, open in a new window)
ReproFF is sutable for keeping human ES/iPS cells in an undifferentiated state without the need of the feeder cells.

Human ES cell line KhES-1 cultured using ReproFF without feeder cells was examined for the morphology (left) and the expression of Oct-3/4, a pluripotency marker gene (right). The cells have grown without any abnormalities. Examined by Kyoto University.
The data sheet for each lot of the product (open in a new window)
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