Freezing Medium for Human ES/iPS Cells (vitrification method)

Increase cell viability up to 10% of input cells (>10-fold higher than DMSO) Rapid freezing by vitrification method using liquid nitrogen The frozen cells can be kept under -135 degC Ideal for serum-free applications Each lot is functionally tested for freezing the cells

Cryopreserving of the cells is a critical step to ensure quick and reliable results when performing stem cell culture. ReproCELL’s freezing medium was formulated by the stem cell expert Prof. Nakatsuji and his colleagues at Kyoto University for human ES/iPS cells. It ensures high levels of viability, up to 10% of the input cells, which is more than 10 times greater than the typical viability obtained with 10% DMSO solution.   Serum-free, Ready-to-use. Store at -80 degC for long term storage.

Figure: Comparison of the survival rate of the cells frozen by using ReproCELL’s freezing solution and 10% DMSO

The instruction manual of Freezing Medium for Human ES/iPS Cells (pdf. open in a new window)
Instruction Movie for freezing and thawing human iPS cells (Jump to the Movie, open in a new window)

Product list

Data Sheets

The data sheet for each lot of the product (open in a new window)