rhFGF-2 (146 a.a.)

Human Recombinant FGF-2 (146 aa)

  • Human recombinant FGF-2 (bFGF), 146 amino acids.
  • Each lot is functionally tested for human iPS cell culture.
  • Available at a discount when purchased with the medium packs.
    (Primate ES Medium, ReproStem, ReproFF, ReproFF2)
FGF-2
bFGF is a critical component of human embryonic and induced pluripotent stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state*. It is typically used at a concentration of 4-5 ng/ml for both feeder dependent and feeder free culture. ReproCELL bFGF is functionally tested to maintain pluripotency in stem cells. Store at -80 degC for long term storage.

*: not necessary for all the cell lines

Instruction Movie for human iPS cell culture using bFGF (Jump to the Movie, open in a new window)

Molecular Mass The 146 amino acid residue recombinant protein has a molecular mass of approximately 16.0K
Concentration 1 mg/mL
Buffer 10 mM Tris-HCL, 1mM DTT, 2M NaCL, pH7.5
Filtered 0.22 μm filter
Purity >97%, as determinded by SDS-PAGE and visualized by CBB stain
Activity The biologocal activity of recombinant human FGF basic was monitored in a mitogenic assay using WST8

 

Price list
Catalog No. Product Retail Price Quantity Purchase
RCHEOT002 bFGF (25ug) $100.00

Contact the distributor of your region
RCHEOT003 bFGF (250ug) $600.00

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Payment method can be chosen from Paypal / credit card / bank transfer.

 

Data Sheets

The data sheet for each lot of the product (open in a new window)